拟南芥蛋白质丰度与基因翻译效率关联分析

A.thaliana Protein Abundance Analysis Coresponding with Elongation Efficiency

蛋白质的合成是一个复杂的过程,其中蛋白质丰度是衡量基因表达的一个最终指标,在生物体生命活动中具有重要作用的蛋白质通常都为高丰度蛋白质。通过对PaxDB网站拟南芥各组织器官蛋白质丰度的统计,并采用DAMBE和CodonW计算其对应基因的I_(TE)和CAI值,最后用R语言分析蛋白质丰度与I_(TE)的关系,并采用对数值替代原有的丰度值。结果表明,所使用的I_(TE)较原有CAI的分析方法更有效,在拟南芥的基因中高表达基因在不同的组织中有相似的表达水平,拟南芥蛋白质丰度与I_(TE)有很好的相关性,并且I_(TE)值能更好地拟合拟南芥蛋白质丰度值的变化。

Protein synthesis is a complex dynamic process,its abundance is a final measurement of gene expression level. Functional important proteins are always highly expressed in most of tissues. The protein abundance of A.thaliana from PaxDB database and computed translation elongation index（ I_（TE） and CAI） of protein coding genes of A. thaliana by both DAMBE and codonW were compiled,and the correlation between protein abundance and translation elongation efficiency was analysed,especially used logarithm in the analysis.The results showed that ITEis better than orignal CAI to analysis,high expressed genes have similar expression level in different tissues in A. thaliana and there was clear correlation between protein abundance and ITEin A.thaliana.