摘要
新一代测序技术(Next-generation sequencing,NGS)在阐明复杂和高度重复的基因组结构,DNA序列与基因组结构变异同重要农艺性状之间的关系等方面具有重要作用。从NGS系统的开发与作物基因组测序,NGS与转录组分析,NGS与全基因组关联图谱,及SNPs开发与预测育种等方面,综述了NGS技术在作物基因组研究中的应用,可为作物基因组研究提供理论基础。
Next-generation sequencing technology plays an important role in elucidating the complex and highly repetitive genome structure,and the relationship between DNA sequence and genomic structural variation with the important agronomic traits. The NGS technology in crop genome research,including the development of the crop genome sequencing NGS technology,NGS and the transcriptome analysis,genome-wide association map and NGS,SNPs development and forecasting breeding,etc. were reviewed. It aims to provide a theoretical basis for crops genomic research.
出处
《中国生物工程杂志》
CAS
CSCD
北大核心
2017年第2期111-120,共10页
China Biotechnology
基金
国家重点研发计划课题(2016YFD0100102-16)
黑龙江省大学生创新创业训练计划重点项目(201610231024)资助项目
关键词
新一代测序
转录组
单核苷酸多态性
作物基因组
Next-generation sequencing
Transcription
Single nucleotide polymorphisms
Crop genomes