木天蓼对角质形成细胞分泌血管内皮生长因子的影响

Effect of Actinidia Polygama on VEGF Expression in Human Epidermal Keratinocytes

目的探讨木天蓼提取物对人表皮角质形成细胞(HEK)产生血管内皮生长因子(VEGF)的影响及木天蓼提取物对HEK增殖作用的影响。方法采用不同浓度、不同种类的木天蓼提取液分别对HEK进行刺激,并在不同时间回收培养液,采用MTT法测定不同时间、不同刺激物作用下HEK增殖活力的变化;采用ELISA技术检测回收的培养液中VEGF的浓度。结果不同浓度、不同种类的木天蓼提取物对HEK的增殖活力均无显著影响(P=0.44);木天蓼的醇溶性提取物(APE)及水溶性提取物(APW)对HEK分泌VEGF均有抑制作用(APE:P<0.01;APW:P=0.02);就抑制HEK分泌VEGF的作用而言,APE作用明显强于APW,差异有统计学意义(P=0.02);APE对HEK分泌VEGF的抑制作用具有浓度及时间依赖性。结论木天蓼提取液能明显抑制HEK分泌VEGF,但对HEK的增殖活力无明显的影响。本研究为研发减轻VEGF引起的红斑的药物提供了一定的理论依据。

Objective To study the effect of actinidia polygama（AP）on expression of VEGF in human epidermal keratinocytes（HEK）.Methods The viability of HEK in all groups was detected by methyl-thiazolyl-tetrazolium（MTT）assay.The concentrations of VEGF in the supernatant were determined by ELISA after the stimulation of the AP extracts.Results The ethanol extract of AP（APE）and the water extract of AP（APW）had no significant effects on the proliferation of HEK in all groups（P=0.44）.Both APE and APW could inhibit the secretion of VEGF in HEK（APE：P〈0.01; APW：P=0.02）, and the biological activity of APE was stronger than that of APW（P=0.02）.The inhibition of APE on the secretion of VEGF by HEK was time-dependent and concentration-dependent.Conclusion Actinidia polygama markedly inhibited the expressions of VEGF in HEK.The results provided some evidence that actinidia polygama could improve the erythema of the sensitive skin.