端粒重复扩增-微孔板杂交法检测妇科肿瘤端粒酶活性的研究

STUDY OF THE TELOMERASE ACTIVITY IN GYNECOLOGIC CARCINOMAS USING TELOMERE REPEAT AMPLIFICATION-MICROWELL PLATE HYBRIDIZATION

目的 :探讨妇科几种常见恶性肿瘤与端粒酶活性的关系。方法 :采用端粒重复扩增 (TRAP)扩增端粒酶产物 ,通过生物素 -亲和素将扩增产物结合于微孔板 ,并与荧光素 (FITC)标记的特异性探针杂交 ,经酶标抗荧光素抗体结合而显色。结果 :妇科恶性肿瘤端粒酶活性检出 2 5例 (89.3% ) ,而肿瘤边缘组织检出 14例 (5 0 .0 % ) ,正常对照组织检出 10例 (35 .7% )。恶性肿瘤端粒酶活性显著高于肿瘤远端组织和正常对照组织 (P<0 .0 5 )。结论 :端粒酶激活可能在妇科恶性肿瘤的发生、发展中起重要作用。 TRAP-微孔板杂交法是检测端粒酶活性灵敏而特异的一种方法。

Objective:To investingate the relationship between telomerase activities and some common gynecologic carcinomas.Method:Telomerase activities were measured by the telomere repeated amplification protocol(TRAP)-microwell plate hybridization system.Result:Telomerase activities in gynecologic carcinomas were found by 89.3% (25/28),in the margin tissues between carcinoma and normal 50% (14/28) and in normal tissues only 35.7%(10/28).Telomerase activities in gynecologic carcinomas were significantly higher than in the margin and normal tissues(P<0 05).Conclusion:Activation of telomerase may play an important role in gynecologic carcinomas.TRAP-microwell plate hybridization system is a specific and sensitive assay to detect the telomerase activity. [