精浆和精子对母兔子宫免疫及孕向发育的影响

The Effect of Seminal Plasma and Sperm on Immunity and Development of Uterus to Pregnancy in Female Rabbits

在结扎子宫颈的情况下,分别在兔同一子宫的两侧子宫角剖腹注入生理盐水(对照)、精浆或精子以研究精浆和精子对子宫免疫和孕向发育的影响。试验共用母兔18只,每组6只。在注入后72 h时处死试验用兔,取子宫进行一般测量,并切片染色,用图象采集分析系统进行研究。结果表明:1)注入子宫的精浆和精子,都能明显降低黏膜中的肥大细胞数量和增加子宫角中冲洗液内的细胞数(P<0.01)。精浆与精子相比,精浆降低黏膜中肥大细胞数量的作用更大(P<0.05);而精子提高子宫角冲洗液内细胞数的作用更强(P<0.01)。2)无论精浆还是精子,都能显著(P<0.05)或极显著(P<0.01)促进子宫(子宫角指数、子宫角长度和子宫角直径)、子宫壁(子宫壁厚度、黏膜层厚度、黏膜上皮层厚度和肌层厚度)、黏膜腺体(腺体数和腺上皮的厚度)以及黏膜血管的孕向发育。精浆与精子相比,就子宫、子宫壁及黏膜血管的孕向发育来说,精浆的作用更明显(P<0.05或P<0.01);而对子宫黏膜腺体孕向发育的作用,精浆和精子间没有明显差异(P>0.05)。

Under the ligation of the cervix, both side of the same uterus in rabbit were injected with saline, seminal plasma and sperm. The effect of seminal plasma and sperm on the immunity and development of uterus to pregnancy were studied. 18 rabbits were used in the experiment, 6 in each group. The rabbits were killed 72h after injection. Uterus from the rabbits were used to section staining, and the organization pictures were analysised by image gathing system. The results are as follows： 1. The seminal plasma and sperm injected into uterus can reduce the number of mast cell （MC） in mucous membrane and increase the number of the cells in uterine flushing fluid （P〈0.01）. The number of MC in seminal plasma group was also significantly less than sperm group （P〈0.05）, but the number of cells in uterine flushing fluid in sperm group was significantly more than in seminal plasma group （P〈0.01）. 2. Whether seminal plasma or sperm, can significantly promote the development of uterine （index of uterine horn, uterine horn length and diameter）, its wall （uterine wall thickness, mucosal thickness, mucosal epithelium thickness, muscle thickness）, mucous glands （ the number of gland, glandular epithelium thickness） and blood vessels （the number of blood vessel in unit area） to pregrancyor P〈0.01）. Comparison between seminal plasma and sperm, the effect of seminal plasma on the development of uterine, uterine wall, mucous blood vessels was greater（P〈0.05 or P〈0.01）, and there is no significant difference between the effect of seminal plasma and sperm on development of the mucoue glands （P〉0.05）.