异丙酚对热打击小鼠氧化应激损伤的保护作用研究

The effect of propofol on anti-oxidative damage in mice induced by heat stress

目的观察热打击小鼠复温后血清中超氧化物歧化酶(SOD)、活性氧(ROS)以及丙二醛(MDA)的变化趋势以及异丙酚对上述指标的干预效应,探讨异丙酚对热打击小鼠氧化应激损伤的保护效应。方法 BALB/c小鼠按随机数字法分为对照组和42℃热打击组,对照组动物始终置于常温(25±0.5)℃,相对湿度(35±5)%环境下;42℃热打击组置于仿真高温气候舱内,舱内温度(35.5±0.5)℃,相对湿度(60±5)%,记录小鼠直肠温度(Tc),当热打击小鼠Tc达42℃移置(25±0.5)℃,(35±5)%相对湿度环境下复温随机分为0 h、3 h、6 h、12 h组(n=3);异丙酚、脂肪乳组于热打击前15 min、100 mg/kg腹腔注射;ELISA法检测血清中SOD、ROS以及MDA含量。结果热打击小鼠血清中SOD浓度在复温后3 h开始降低,至复温后12 h其值达最低,而血清中ROS与MDA浓度均在复温后3 h开始升高,至复温后12 h其值达最高,与对照组比较差异均有统计学意义(P<0.05);热打击小鼠血清中SOD浓度与ROS、MDA表达呈显著的负相关;使用异丙酚预处理小鼠,可以明显上调热打击小鼠血清中SOD表达,降低ROS和MDA水平,与热打击组比较差异均有统计学意义(P<0.05)。结论异丙酚可以通过上调热打击小鼠血清中SOD表达,降低ROS和MDA水平,从而促进机体内氧化与抗氧化系统的动态平衡恢复发挥抗氧化损伤效应。

Objective To investigate the changes of SOD、ROS and MDA in the serum and the effect of propofol on the above indexes in order to explore the protection effect of propofol on oxidative damage in miace induced by heat stress. Methods BALB / c mice were randomly divided into a control and a heat stress group（ 42℃）,the former placed at a temperature of（ 25. 0 ± 0. 5） ℃ and a relative humidity of（ 35 ± 5） % and the latter in a simulated incubator at（ 35. 5 ± 0. 5） ℃ and a relative humidity of（ 60 ± 5） %. When the rectal temperature reached 42℃,the animals were removed from the incubator and cooled at an ambient temperature of（ 25. 0 ± 0. 5） ℃ and a humidity of（ 35 ± 5） % for 0,3,6 and 12h（ n=3）. Measurement of the SOD,ROS and MDA levels in the serum were tested by ELISA. Results Compared with those in the control group,the concentrations of SOD were dramatically decreased at 3h and the value of the temperature reached the lowest at 12 h after rewarming;while the levels of serum ROS and MDA were increased markedly at 3h and the value of the temperature reached the highest at 12 h after rewarming（ P〈0.05）. The concentrations of SOD and the expression of MDA,ROS in the serum of mice with heat stress showed significant negative correlation. Compared with the heat stress group,propofol can significantly up-regulate the expression of SOD,reduce the level of ROS and MDA in the serum of heat stress mice,the differences were statistically significant（ P〈0.05）. Conclusion Propofol might via up-regulate the expression of SOD and reduce the level of ROS and MDA in the serum of heat stress mice to promote the dynamic balance recovery of oxidation and anti-oxidation system. Thus,propofol has a role of anti-oxidative damage during heat stress.