丝状真菌启动子的基因工程改造进展

Research Progress Towards Promoters Genetic Engineering in Filamentous Fungi

丝状真菌是重要的工业酶生产菌,具有优异的蛋白表达和分泌能力,如里氏木霉在工业发酵中其内源纤维素酶产量可高达100g/L。目前,丝状真菌已被成功用于表达外源基因,但与内源基因相比,外源基因的表达效率常常不高。使用强启动子来驱动基因转录可有效提高外源基因的表达水平,因此本文就通过基因工程的方法对丝状真菌的启动子进行改造以提高内、外源基因的转录效率的研究进行总结与展望。

Filamentous fungi are important industrial microbes with excellent ability of protein expression and secretion.For example,Trichoderma reesei can produce its endogenouscellulase with a titer of up to 100g/L in industrial fermentation conditions.Currently,filamentous fungi have been successfully used to express heterologous genes.However,compared with endogenous gene expression,the expression of heterologous genes in filamentous fungi is often of low efficiency.In order to increase the expression level of heterologous genes,one effective way is to use strong promoters to drive gene transcription.In this paper,we discuss the progress of genetic modification of the filamentous fungi promoters to improve transcription efficiency of endogenous and heterologous genes.