摘要
目的:观察小干扰RNA(siRNA)介导的胰岛素样生长因子1受体(IGF-1R)基因沉默对肾癌细胞786-0增殖、迁移、侵袭和细胞周期的影响。方法:采用实时定量PCR(real-time PCR)和蛋白印迹法(Western blot)测定转染后肾癌细胞IGF-1R及下游相关产物的表达;采用Cell counting kit-8(CCK-8)检测细胞增殖能力;Transwell实验及划痕实验检测细胞迁移及侵袭能力;流式细胞仪检测细胞凋亡及周期改变。结果:IGF-1R基因的siRNA可有效抑制IGF-1R mRNA及蛋白的表达(P<0.01);细胞增殖实验结果显示IGF-1R-siRNA转染组增殖能力显著减弱(P<0.01);Transwell实验及划痕实验结果显示IGF-1R-siRNA转染组侵袭及迁移能力明显降低(P<0.01);流式细胞术检测到转染后细胞G1期细胞数明显升高,发生G1/S期阻滞(P<0.05);IGF-1R下游蛋白p-IRS1、p-IRS2、p-SHC水平显著降低。结论:IGF-1R表达下调可以抑制肾癌786-0细胞的生长、增殖、迁移和侵袭的能力,并可以使肾癌786-0细胞G1/S期细胞周期停滞。
Objective: To explore the effects of small interference RNA(siRNA)-mediated knockdown of IGF-1R on cell growth,proliferation,invasion,migration and cell cycle phase G1/ S transition in 786-0 RCC cells.Methods:Real-time PCR and Western blot were used to detect the expression of IGF-1R and the downstream proteins of IGF-1R pathway.Cell proliferation ability was determined by CCK-8 method,the change of cell cycle was detected by flow cytometry.Results: RNA interference targeting insulin-like growth factor receptor 1(IGF-1R) could effectively inhibit the expression of mRNA and protein of IGF-1R(P〈0.01).Cell proliferation assay showed that the proliferation of transfected 786-0 cells were significantly decreased(P〈0.01).Transwell assay and Wound-healing assay revealed that the abilities of migration and invasion were significantly decreased in 786-0 cells transfected with IGF-1R-siRNA(P〈0.01).The transfected 786-0 cells had a significant increase in G1-phase population(p〈0.05).Western blotting showed that 786-0 cells transfected with IGF-1R-siRNA decreased the expression of p-IRS1,p-IRS2 and p-SHC.Conclusion: The down-regulation of IGF-1R suppressed the proliferation,migration and invasion and induces G1/ S phase cell cycle arrest in 786-0 RCC cells.
出处
《现代肿瘤医学》
CAS
2017年第8期1208-1213,共6页
Journal of Modern Oncology
关键词
胰岛素样生长因子-1受体
肾细胞癌
小干扰RNA
insulin-like growth factor receptor 1
renal cell carcinoma
small interference RNA