施氮和水分管理对光合碳在土壤-水稻系统间分配的量化研究

Input and Distribution of Photosynthesized Carbon in Soil-Rice System Affected by Water Management and Nitrogen Fertilization

施肥和水分管理是影响水稻生长的两个关键因素,探讨水肥耦合条件下光合碳在"水稻-土壤"系统输入与分配动态,对深入解析稻田土壤碳循环具有重要意义.本研究通过盆栽试验,选用籼性常规水稻品种(中早39),采用13C-CO_2连续标记技术,量化研究施氮和水分管理对光合碳在"水稻-土壤"系统中分配的影响.结果表明:施氮增加了水稻地上部干物质重和碳、氮含量,却显著降低了水稻根冠比;干湿交替使施氮条件下的水稻地上部全碳、全氮含量较持续淹水处理分别提高了22%和33%,根系中全碳、全氮含量分别提高了36%、44%,这表明施氮有利于水稻地上部生长,而干湿交替显著促进了水稻根系的生长.施氮显著增加水稻地上部13C含量,与不施氮处理相比增加了32%~83%;施氮使光合碳在水稻地上部的回收率增加6%~32%,在根系的回收率减少18%~59%.水分管理对13C分配的影响表现为:连续标记22 d后,在施氮条件下,干湿交替使水稻地上部、根系13C量均有一定量的增加;不施氮条件下,干湿交替与持续淹水处理相比,地上部13C量减少10.3mg·pot-1,回收率降低了11%~12%;根系13C量增加1.9 mg·pot-1,回收率提高了24%~57%.施氮和干湿交替都显著增加了13C在根际土壤中的累积与回收率.因此,施氮增加了光合碳在土壤-水稻系统中的累积,但降低了光合碳在根系中的分配,干湿交替比持续淹水更利于光合碳向土壤中的传输与累积,水肥耦合管理显著调控了光合碳的传输与分配.该研究进一步量化了水肥管理条件下水稻光合碳的分配及其在土壤有机碳库中的传输特征,为稻田水肥管理、水稻土有机质积累持续机制提供了理论依据和数据支撑.

Fertilizer and water management are two key factors for rice growth. A better understanding of the carbon（ C） cycling in paddy soil requires investigation into the input characteristics and distribution dynamics of photosynthesized carbon in rice-soil system.We grew rice（ Zhongzao 39） in PVC pots and used the13C-CO2 continuous labeling method to quantify the allocation of photosynthesized carbon in rice-soil system under two regimes（ Drying-rewetting vs. continuous watering） and N fertilization（ 250mg·kg- 1 vs. no addition）. The results showed that nitrogen fertilizer application increased rice shoot biomass and the amount of C and N,but had no significant influence on rice root biomass. Thus,nitrogen fertilizer application decreased rice biomass root / shoot ratio significantly. Drying-rewetting with N fertilizer treatment resulted in higher total C and N amount by 22% and 33%,respectively,in the shoot,and by 36% and 44%,respectively in the root than continuous watering with nitrogen fertilizer treatment. These results indicated that nitrogen fertilizer application promoted the growth of rice shoot. Nitrogen fertilizer application significantly increased the13 C content in rice shoot by 32%-83% over the control without N addition. Nitrogen fertilizer application also increased the13 C recovery in rice shoot by 6%-32%,but decreased that in the root by 18%-59%. Pertaining to water effect,drying-rewetting with N application increased the amount of13 C in rice shoot and root. However,without N addition,the amount and the recovery of13 C in shoot dropped by 10. 3 mg·pot- 1and 12%,respectively,compared with the continuous watering treatment. The root,on the other hand,recorded increases in both the amount and the recovery of13 C by 1. 9 mg·pot- 1and 57%,respectively. Furthermore,the deposition ofassimilated C into rhizosphere-soil increased by both the individual and the interactive effects of N fertilizer application and dryingrewetting treatment. Thus,combining N fertilizer and drying-rewetting water management led to more increased allocation and deposition of photosynthesized carbon in soil-rice system compared with combined continuous flooding and N application. This study was able to quantify the partitioning and allocation of rice photosynthesized carbon into different plant and soil pools under different water and N fertilizer treatments,and can serve as a useful guide for better water and nutrient management practices in paddy-rice production that can achieve both sustainable high yield and sequestration of more C within the paddy soil system.