B7-H4蛋白在卵巢子宫内膜样腺癌组织中的表达及临床病理意义

Expression and Significance of B7-H4 in Endometrioid Ovarian Adenocarcinoma

目的探讨B7-H4蛋白在卵巢子宫内膜样腺癌组织中表达及临床病理意义。方法应用免疫组织化学Elivision法检测30例卵巢子宫内膜样腺癌、15例卵巢子宫内膜交界瘤、30例卵巢子宫内膜异位症及20例正常卵巢组织中B7-H4蛋白表达,分析其表达差异与患者临床病理特征关系及与患者术前血清CA125水平相关性。结果B7-H4蛋白在卵巢子宫内膜样腺癌、交界瘤、内膜异位症及正常卵巢组织中阳性表达率分别为96.7%(29/30)、53.3%(8/15)、30.0%(9/30)和5.0%(1/20),阳性率逐级下降,差异有统计学意义(P=0.000<0.05);B7-H4蛋白表达与卵巢子宫内膜样腺癌患者年龄无关(P>0.05),表达强度与临床分期、病理分级相关(P<0.05),并与术前血清CA125水平呈正相关,B7-H4蛋白高表达患者术后易发生癌灶残留。结论 B7-H4蛋白在卵巢子宫内膜样腺癌组织中弥漫性高表达,表达强度与肿瘤发展阶段及级别相关,提示B7-H4分子在卵巢子宫内膜样腺癌发生、发展中起重要作用;B7-H4蛋白表达强度与患者术前血清CA125水平呈正相关。B7-H4蛋白检测有助于卵巢子宫内膜样腺癌早期诊断。

Objective To explore the expression and clinicopathologic significance of B7-H4 in endometrioid ovarian adenocarcinoma.Methods The expression of B7-H4 was determined by using immunohistochemical Elivision method in 30 cases of endometrioid ovarian adenocarcinoma,15 cases of borderline endometrioid tumor of the ovarian,30 cases of ovarian endometriosis,and 20 normal ovarian tissues.The relationships of B7-H4 expression to clinicopathologic features and preoperative serum CA125 levels were analyzed.Results The positive expression rates of B7-H4 in endometrioid ovarian adenocarcinoma,borderline endometrioid tumor,ovarian endometriosis and normal ovarian tissues were 96.7%(29/30),53.3%(8/15),30.0%(9/30)and 5.0%(1/20),respectively.Differences were significant between endometrioid ovarian adenocarcinoma and other ovarian tissues(P<0.05).The expression of B7-H4 protein was not associated with the age of patients with endometrioid ovarian adenocarcinoma(P>0.05),but was correlated with the clinical stage and pathological grade(P<0.05).Furthermore,there was a positive correlation between B7-H4 expression and preoperative serum CA125 levels.Moreover,the occurrence of postoperative residual foci was related to the high expression of B7-H4.Conclusion B7-H4 is diffusely and highly expressed in endometrioid ovarian adenocarcinoma,and the expression of B7-H4 is associated with the stage and grade of the tumor,suggesting that B7-H4 play an important role in the occurrence and development of endometrioid ovarian adenocarcinoma.In addition,B7-H4 protein expression is positively correlated with the preoperative serum CA125 levels.Therefore,the detection of B7-H4 protein is conducive to the early diagnosis of early diagnosis.