Cyclin D1在疣状胃炎、胃息肉、胃癌中的表达和幽门螺杆菌感染的意义

The clinica significance of expression of cyclind1 and helicobacter pylori infection in verrucous gastritis gastric poly and gastritis cancer

目的检测细胞周期蛋白D1(Cyclin D1)在不同分型疣状胃炎、胃息肉、胃癌组织中的表达水平和幽门螺杆菌的感染率,寻求判断不同分型疣状胃炎向胃癌发生的指标。方法收集内蒙古包钢医院2012年1~12月份病理和内窥镜结合确诊的病例,通过免疫组织化学染色SP法,检测Cyclin D1在慢性浅表性胃炎30例、疣状胃炎非成熟型30例、疣状胃炎成熟型30例、腺瘤性胃息肉25例、胃癌30例组织中的表达水平,通过快速尿素酶法和C14—UBT法检测H.pylori(Hp)的感染情况。结果慢性浅表性胃炎、疣状胃炎非成熟型、疣状胃炎成熟型、腺瘤性胃息肉、胃癌组织中Cyclin D1的阳性表达率分别为(10%、13.39%、36.74%、44%、60%)呈逐渐升高趋势。成熟型疣状胃炎,腺瘤性胃息肉、胃癌组与慢性浅表性胃炎、疣状胃炎非成熟型组的阳性率比有差异(P﹤0.05),而疣状胃炎成熟型、腺瘤性胃息肉、胃癌组之间Cyclin D1的阳性表达率无统计学差异(P﹥0.05)。Hp在慢性浅表性胃炎、疣状胃炎非成熟型、疣状胃炎成熟型、腺瘤性胃息肉、胃癌中的阳性表达率(26.7%、33.3%、66.7%、72%、75%)呈逐渐升高趋势,经统计学分析,疣状胃炎成熟型、腺瘤性胃息肉、胃癌与慢性浅表性胃炎、疣状胃炎非成熟型组相比,Hp的感染率有差异(P﹤0.05),而疣状胃炎成熟型、腺瘤性息肉、胃癌组,Hp感染率无差异。结论 Cyclin D1蛋白的阳性表达在慢性浅表性胃炎、非成熟型疣状胃炎之间无差异,在非成熟型疣状胃炎和成熟型疣状胃炎之间有差异,Cyclin D1蛋白的阳性表达在成熟型疣状胃炎、腺瘤性胃息肉、胃癌组织中表达无统计学差异,提示成熟型疣状胃炎已具有癌变的分子生物学特征。Hp感染在疣状胃炎成熟型、腺瘤性胃息肉、胃癌组之间无统计学差异,在慢性浅表性胃炎、非成熟型疣状胃炎与疣状胃炎成熟型、腺瘤性胃息肉、胃癌组之间有统计学差异。在Hp感染阳性的患者中Cyclin D1阳性表达明显高于Hp感染阴性组的患者,且具有统计学差异,提示在成熟型疣状胃炎向胃癌的发生过程中Hp感染与Cyclin D1起到了协同作用。

Objective This research was designed to study the expression of cell cycle protein DI （CyclinD1） in verrucous gastritis, gastric poly and gastritis cancer and seek the early diagnostic indicator of molecular biology in the gastric carcinoma. Methods Tissue specimens used for this study were obtained from 145 patients, which were resected surgically or endoscopically at the Third Affiliated Hospital of XXX medical university from January. 2012 to December. 2012. The immunohistochemical SP was used to examine the expression of CyclinD1 in different tissue.3. Through rapid urea enzymatic and CI3-UBT method to detect H.p ylori （Hp） infection. Results In the specimens of chronic superficial gastritis, verrucous gastritis without mature, verrucous gastritis with mature, gastric poly and gastric carcinoma, The positive expression rates of CyelinD1 was 10%, 13.3%, 36.7%, 44%, 60%.The expression of CyclinD I was difference when erracous gastritis with mature, gastric poly and gastric carcinoma respectively compared with chronic superficial gastritis and verrucous gastritis without mature （P 〈 0.05）. The expression of CyclinD1 was not difference between in errucous gastritis with mature, gastric poly and gastric carcinoma.The positive expression rates of Hp inection is 26.7%, 33.3% , 66.7%, 72%, 75%. The expression of Hp was difference when errucous gastritis with mature, gastric poly and gastric carcinoma respectively compared with chronic superficial gastritis and verrucous gastritis without mature （P 〈 0.05）. The expression of lip was not difference between in errucous gastritis with mature, gastric poly and gastric carcinoma. Conclusion No significant difference was observed for the expression of CyclinD1 between chronic superficial gastritis and verrucous gastritis without mature. But the expression of CyclinD1 was significant difference when errucous gastritis with mature compared with verrucous gastritis without mature. The expression of CyclinD1 was not significant difference between in errucous gastritis with mature, gastric poly and gastric carcinoma, verrueous gastritis with mature has the character of cancerous molecular biology. The expression of Hp was not significant difference between in errueous gastritis with mature~ gastric poly and gastric carcinoma. The expression of HP was significant difference when errucous gastritis with mature, gastric poly and gastric carcinoma respectively compared with chronic superficial gastritis and verrueous gastritis without mature. The expression of CyclinD1 in Hp infection postive patients is higher than the expression of CyclinD1 in HP infection negative patients. It is significant different. Hp infection and the expression of CyclinD1 make the progress verrucous gastritis with mature to the gastritis cancer. By detecting the positive expression of CyclinD1 and Hp infection can be used as a diagnostic and assessment of verrucous gastritis to gastric cancer occurrence and development indicators, verrucous gastritis with mature could be cancer, It should be treated.