HBsAg阳性母亲新生儿TLR3蛋白表达水平检测分析

Detection and analysis of TLR3 protein expression levels in the neonates born by mothers with positive HBsAg

目的分析HBs Ag阳性母亲新生儿HBV宫内传播与TLR3蛋白表达水平的关系。方法连续收集2011-2014年太原市第三人民医院妇产科HBs Ag阳性母亲及分娩新生儿222例作为研究对象,采集HBs Ag阳性母亲分娩前肘静脉血,足月分娩后纳入本研究;新生儿24 h内注射乙肝疫苗和乙肝高效价免疫球蛋白(HBIG)前无菌釆集股静脉血抗凝血和非抗凝血各3 ml,4℃冷藏保存;采用面对面调查方法收集母儿的流行病学资料。结果 HBs Ag阳性母亲分娩新生儿TLR3测定分析,HBV宫内传播组新生儿TLR3平均荧光强度为(64.89±3.09)%,高于非宫内传播组新生儿(29.38±5.78)%,但两组差异无统计学意义(P>0.05)。结论 HBV宫内传播组新生儿TLR3蛋白含量高于非宫内传播组新生儿,由于宫内传播组新生儿受到HBV的刺激TLR3蛋白含量升高,TLR3蛋白可能在HBV宫内传播发生机制中起重要作用。

Objective To analyze the relationship between intrauterine transmission of hepatitis B virus （HBV） in the neonates born by mothers with positive HbsAg and the expression levels of TLR3 protein. Methods A total of 222 neonates and their mothers with positive HbsAg were selected from Department of Gynecology and Obstetrics in the Third People＇s Hospital of Taiyuan from 2011 to 2014 were collected as study objects, elbow venous blood specimens of the mothers were collected befnre delivery, then the mothers were enrolled in this study after term delivery. Femoral venous anticoagulant blood （3 ml） and non-anticoagulant blood （3 ml） （4℃ cold storage） were obtained before neonatal injection of hepatitis B vaccine and hepatitis B immunoglobulin （HBIG） within 24 hours after birth. A face-to-face survey was used to collect the epidemiological data of mothers and children. Results The mean fluorescence intensity of neonatal TLR3 protein in HBV intrauterine transmission group was （ 64. 89 ± 3.09 ） % , which was higher than that in non - intrauterine transmission group [ （ 29. 38 ± 5.78 ） % ] （ P〉0. 05 ） . Conclusion TLR3 protein content in HBV intrauterine transmission group is higher than that in non-intrauterine transmission group, which may be due to HBV stimulation, TLR3 protein may play an important role in occurrence of HBV intrauterine transmission.