利咽灵对慢性咽炎大鼠治疗作用的实验研究

Experimental Study of Therapeutic Effect of Liyanling Granules on Chronic Pharyngitis in Rats

目的:研究利咽灵颗粒对慢性咽炎的治疗作用。方法:建立大鼠慢性咽炎模型,连续灌胃给药7 d。观察利咽灵颗粒对慢性咽炎大鼠咽部组织病理形态学、前列腺素(PGE_2)、白细胞介素-1β(IL-1β)及肿瘤坏死因子(TNF-α)含量的影响;观察利咽灵颗粒对大鼠全血黏度、血浆黏度的影响。采用角叉菜胶致大鼠足肿胀和琼脂肉芽增生方法,观察其抗炎作用。结果:与模型组比较,利咽灵颗粒可明显降低大鼠咽部组织中的PGE_2、IL-1β与TNF-α水平(P<0.05或P<0.01);可明显降低血液黏度和血浆黏度(P<0.05或P<0.01);改善模型大鼠咽部组织病理形态学(P<0.05或P<0.01)。与空白对照组比较,能抑制角叉菜胶所致大鼠足肿胀率、减轻琼脂肉芽肿质量(P<0.05或P<0.01)。结论:利咽灵颗粒对大鼠慢性咽炎有较好的治疗和修复作用。其可能通过抑制炎症组织细胞释放PGE_2、IL-1β和TNF-α,改善局部血液流变起到治疗作用。

Objective ： To study the therapeutic effect of Liyanling granules on chronic pharyngitis. Methods ： The chronic pharyn- gitis rat models were established, and administered orally for 7 days. The influence of Liyanling granules on the pathology of pharyngeal tissue and the contents of PGE2, IL-1β and TNF-α were observed. The blood was withdrawn to detect the whole blood viscosity and plasma viscosity, and the anti-inflammatory effects were observed in two kinds of rat models ： foot pad swelling induced by carrageenin and agar-induced chronic granuloma. Results ： Liyanling granule could significantly decrease the levels of PGE2, IL-1β and TNF-α（P 〈 0.05 or P 〈 0.01 ） and the viscosity of blood and plasma（ P 〈 0.05 or P 〈 0.01 ）, improve the pathological morphology of rat pharynge- al tissues（ P 〈 0.05 or P 〈 0.01 ）, inhibit the swelling of foot pad induced by carrageenin and the granuloma induced by agar in rats（ P 〈0.05 or P 〈0.01 ）. Conclusion： Liyanling granules has a certain therapeutic effect on chronic pharyngitis in rats, and the mecha- nism may be related with the inhibition of inflammatory cytokines PGE2, IL-1β and TNF-α and the improvement of blood theology.