新乡市268例HIV/AIDS病人样本的流式细胞仪检测数据分析

Analysis of testing results of 268 HIV/AIDS samples by flow cytometry

目的了解河南省艾滋病(AIDS)流式细胞仪实验室间、检测试剂间的能力差异。方法在河南省和新乡市的两家流式细胞仪检测实验室,同时对268例艾滋病病毒(HIV)感染者/AIDS病人(简称HIV/AIDS病人)的血液样本分别进行T淋巴细胞亚群检测,对比分析各组T淋巴细胞亚群检测数据总体、实验室间、试剂间的差异。结果检测结果总体比较显示,CD3、CD8、CD4、CD4/CD8各参数差异均无统计学意义(P>0.05)。实验室间检测能力比较发现,新乡市、河南省两个流式细胞仪实验室CD4^+T淋巴细胞分别为432(267~623)个/μL、507(276~698)个/μL,差异有统计学意义(Z=2.21,P=0.03)。而CD3、CD8及CD4/CD8其他T淋巴细胞亚群差异无统计学意义(P>0.05)。进口试剂与国产试剂检测结果差异无统计学意义(P>0.05)。结论河南省各流式细胞仪实验室间检测能力存在部分差异,有待继续加强室间考评的频度和力度,及时发现存在的问题,进一步提高流式细胞仪检测质量。

Objective To learn the testing capacity difference of acquired immune deficiency syndrome（AIDS）by flow cytometry in laboratories and reagents in Henan province.Methods 268 of human immunodeficiency virus（HIV）/AIDS samples were collected for detection of T lymphocyte subsets in Xinxiang city and Henan provincial laboratories respectively.Then the differences were compared between the laboratories and reagents of T lymphocyte subsets.Results The testing results of CD3,CD8,CD4,and CD4/CD8 showed no statistically significant difference（P〉0.05）.The results of CD4~＋T counts tested by the two flow cytometry laboratories in Xinxiang and Henan were 432（267-623）/ul and 507（276-698）/ul respectively.The difference was statistically significant（Z=2.21,P=0.03）.There was no significant difference in the testing results of CD3,CD8 and CD4/CD8 T lymphocyte subsets（P〉0.05）,as well as that between imported and domestic reagents（P〈0.05）.Conclusion The flow cytometry laboratories showed different testing capacity in Henan province.Monitoring and evaluation should be strengthened to improve the quality of flow cytometry detection.