正交试验法优选首乌颗粒的水提醇沉工艺

Optimization of Water Extraction and Alcohol Precipitation Technology for Shouwu Granula by Orthogonal Test

目的优选首乌颗粒水提醇沉工艺。方法以二苯乙烯苷含量和干膏率的综合评分为指标,采用正交试验法,考察加水量、提取次数、提取时间对水提工艺的影响;考察水提液相对密度、醇沉浓度、静置时间对醇沉工艺的影响。结果优选水提工艺:加12倍量水,提取3次,每次1 h;优选醇沉工艺:水提液浓缩至相对密度1.10(60℃),醇沉浓度60%,静置48 h。结论优选的工艺稳定可行,可用于制剂的工业化生产。

Objective To optimize water extraction and alcohol precipitation technology of Shouwu granula.Methods With composite score of the content of Stilbenes and dry extract yield as index,effects of the amount of water,extraction times and time on extraction technology were investigated by orthogonal test;the relatively density of concentrated liquid,the concentration of alcohol precipitation and storage time were chosen as factors,alcohol precipitation technology was optimized by orthogonal test.Results Optimum extraction process was as following：extracted 3 times with 12 times the amount of water,1 hour each time;Optimized alcohol precipitation technology was：the relative density of concentrated liquid 1.10（60）,the concentration of alcohol precipitation 60%,placed 48 hours.Conclusion The optimization method is simple and stable,and is suitable for industrial production.