RNAi慢病毒表达对小鼠海马CA1神经元电生理功能的影响

The effect of expression of RNA interfering lentivirus in the hippocampus of mice on the electrophysiological functions of hippocampal CA1 pyramidal neurons

采用立体定位注射方法使小鼠海马脑区CA1区域感染以U6为启动子表达EGFP（pU6-EGFP）的特异性感染神经元的RNA干扰（RNAi）慢病毒颗粒（pU6-EGFP）,2~3周后在离体海马脑片上应用膜片钳与场电位记录方法研究CA1锥体神经元在电生理特性、突触传递以及突触可塑性方面的变化。结果表明：pU6-EGFP慢病毒注射后未对海马Schaffer collateral-CA1基本突触传递功能、突触可塑性功能、锥体神经元膜电位及放电特性（静息膜电位、动作电位阈值、可以诱发动作电位的阈电流）产生影响。说明pU6-EGFP慢病毒注射后对小鼠海马脑片细胞电生理功能没有产生影响,因此应用其脑内注射方法进行中枢神经系统功能学领域的研究是可行的。

U6promoter and enhanced green fluorescent protein（pU6-EGFP）-containing RNA interfering lentivirus was injected into the hippocampal CA1 region for 2-3weeks by the stereotaxic,then we used patch clamp and field potentials to study the changes in the electrophysiological property,synaptic transmission and synaptic plasticity of hippocampal CA1 pyramidal neurons.The result showed that：pU6EGFP lentivirus injection did not affect the basal synaptic transmission,the synaptic plasticity,the membrane potential and the discharge characteristics of CA1 pyramidal neurons（resting membrane potential,threshold of action potential,the current to evoke action potential）in the Schaffer collateral-CA1 pathway.The data demonstrated that pU6-EGFP lentivirus injection had no effect on the electrophysiological functions of hippocampal slices,so it is a feasible strategy to apply the pU6-EGFP lentivirus in the functional study of central nervous system.