聚甲基丙烯酸缩水甘油酯预浸润石英纤维的细胞毒性和生物相容性研究

Cytotoxicity and biocompatibility of PGMA pre-impregnated quartz fiber

目的 对聚甲基丙烯酸缩水甘油酯（PGMA）预浸润石英纤维进行体外细胞毒性和生物相容性研究,以便对该材料应用于口腔临床提供实验依据。方法 使用CCK8法测定小鼠成纤维细胞（L929）以及小鼠牙龈上皮细胞（GE1）在不同材料（非聚合状态PGMA预浸润石英纤维、聚合状态PGMA预浸润石英纤维）浸提液中培养2、4、7 d的吸光度（A值）,计算细胞相对增殖率（RGR）,判断细胞毒性的级别。结果 非聚合状态PGMA预浸润石英纤维100%浸提液、50%浸提液细胞组在不同时间点相对增殖率为44.59%-65.66%（L929）和52.06%-62.86%（GE1）,其细胞毒性程度为2-3级。而聚合状态PGMA预浸润石英纤维100%浸提液、50%浸提液细胞组的相对增殖率为90.56%-100.89%（L929）和89.99%-101.21%（GE1）,其细胞毒性为0-1级。结论 聚合状态PGMA预浸润石英纤维安全可靠,具有良好的临床应用前景。

Objective To evaluate the cytotoxicity, biocompatibility and biological safety quartz fiber in vitro, providing scientific basis for clinical application. Methods of PGMA pre-impregnated According to standard of GBT16886.5-2003 and YYT0268-2008 documents, the cellular cultivation and cytotoxicity test in vitro were conducted to evaluate the target materials on the morphology, growth and proliferation of cultured cells （L929 and GEl ）. ResuLts The range of cell relative growth rate （RGR） of 50% and 100% extraction of non-polymerized composite were 44.59%-65.66% （L929） and 52.06%-62.86% （GEl） , and the cytotoxieity was grade 2-3. The range of cell relative growth rate （RGR） of 50% and 100% extraction of polymerized composite were 90.56%-100.89% （L929） and 89.99%-101.21% （GEl） , and the cytotoxicity was grade 0-1. Conclusion Polymerized PGMA pre-impregnated quartz fiber is safe and fit for the clinical application.