CGI-58基因条件敲除对小鼠中枢神经系统功能的影响

Effects of comparative gene identification-58(CGI-58) on the central nervous system

目的:探讨comparative gene identification-58(CGI-58)基因失功能对小鼠中枢神经系统功能的影响。方法:选择中枢神经系统星形胶质细胞中特异性敲除CGI-58的小鼠(CGI-58KO)为研究对象,通过Morris水迷宫、Y-迷宫、转杆实验、尾部及足底的痛觉测试,比较其与野生型小鼠在运动协调性、空间学习记忆及感觉能力等方面的差别。建立小鼠脊髓损伤模型,在损伤后不同时间点,采用小鼠后肢运动功能评分(basso mouse scale for locomotion,BMS)和痛觉测试检测,比较CGI-58KO小鼠与CGI-58野生型小鼠在神经运动功能与感觉功能恢复情况的差别。结果:与野生型小鼠相比,CGI-58KO小鼠在Y-迷宫中找到安全区的用时较长,在转杆实验中停留在转杆上的时间较短,对痛觉的反应时间较长,在Morris水迷宫中找到安全平台用的时间和游泳的距离都较长;在脊髓损伤后,与野生型小鼠相比,CGI-58KO小鼠的后肢运动功能BMS评分较低,对痛觉的反应时间较长。结论:在小鼠神经系统胶质细胞中CGI-58基因的失功能显著影响了小鼠运动、学习记忆、空间认知和感觉能力,推测该基因在小鼠神经系统功能中具有重要的作用。

Objective：To explore the influence of the loss of comparative gene identification-58（CGI-58） on mice’s central nervous system function. Metholds： Choose the CGI-58KO mice as the research object, in whose glia cells the CGI-58 has been knocked out specifically. Through Morris Water Maze, Y-maze, rotarod test and the pain test in tails and plantars, compare the differences between them and wild type mice in motor coordination and spatial learning memory and sensibility. Spinal cord injury in mice model was established , adopt basso mouse scale for locomotion （ BMS ） score and pain test at different time points after their injuring, and compare the differences between CGI-58KO mice and CGI-58 wild type mice in the recovery of neuromotor function and feeling function. Results： Compared with wild type mice, it takes CGI-58KO mice to＆amp;nbsp;find safety place much longer time in Y-maze, shorter time to stay on the rotarod in the Rotarod test, longer time for pain response, longer time to find safety platform in Morris Water Maze and farther swimming distance. After the spinal cord injured, the BMS score of CGI-58KO mice’s hindlimb motor functions is lower and the sensibility response time to pain is longer, compared with the CGI-58 wild type mice. Conclusion：This research indicates that the loss function of CGI-58 genes in mice’s glia cells significantly affected the mouse movement ability, learning and memory, space cognitive and sensibility to pain. It is predicted that the genes play an important role in the nervous system function in mice.