疏风宣肺解毒方对流感病毒性肺炎小鼠基因表达谱的影响

Effects of Shufeng Xuanfei Jiedu formula on whole genome expression profiles of pneumonia mice infected with influenza virus

目的:采用基因表达谱芯片技术筛选疏风宣肺解毒方治疗流感病毒感染小鼠相关的差异表达基因,初步探讨方药治疗流感的作用机制。方法:流感病毒肺适应株FM1滴鼻感染小鼠,建立小鼠流感病毒性肺炎模型,方药灌胃治疗4 d,提取肺组织总RNA,与小鼠全基因表达谱芯片杂交后进行检测。对筛选得到的药物治疗过程中的差异表达基因进行生物信息学分析。结果:基因芯片结果显示,与模型组相比,给药组表达量发生变化的基因中上调的有2 659条,下调的有2 216条。结论:疏风宣肺解毒方作用涉及免疫学方面的分子功能为:胞内信号级联放大效应,浆膜、免疫应答、细胞增生的调节,免疫防御及细胞程序性死亡等;主要调节的代谢途径有:丝裂原活化蛋白激酶信号通路、细胞因子与细胞因子受体的相互作用、肿瘤信号通路、toll样受体信号通路、信号转导及转录激活因子途径、自然杀伤细胞介导的细胞毒作用及趋化因子信号通路等。

Objective：To study the effects of Shufeng Xuanfei Jiedu Formula on whole expression profiles of mice infected with influenza virus FM1 by the gene chip technology,and analyze the mechanism of the formula on gene level.Methods：Established the model of Pneumonia by nasal dropping influenza virus A in mice. Total RNA was extracted in each groups. Then gene chips were used to screen these RNA samples. Calculated the probe signal intensity ratio in each group vs model group. Results：By Shufeng Xuanfei Jiedu formula group compared with the model group,2 659 genes were upregulated,2 216 genes were down-regulated. Conclusion：The mechanism of Shufeng Xuanfei Jiedu formula involves the immunology molecular function：intracellular signaling cascade,plasma membrane,immune response,regulation of cell proliferation,defense response,regulation of apoptosis,etc. The metabolic pathways regulated by Shufeng Xuefei Jiedu formula involve：MAPK signaling pathway,Cytokine-cytokine receptor interaction,pathways in cancer,Toll-like receptor signaling pathway,Jak-STAT signaling pathway,Natural killer cell mediated cytotoxicity,chemokine signaling pathway,etc.