红松不同种源的遗传多样性分析

Study on the Genetic Diversity of Pinus Koraiensis in Different Provenances

红松是小兴安岭林区的主要建群树种,在小兴安岭总体生态系统中具有重要的生态地位。为了保证小兴安岭林区红松的遗传丰富度,为小兴安岭林区提供优质的红松造林苗木。研究运用10个经过筛选后的引物,用ISSR分析方法对10个不同红松天然林种源进行了分子标记分析。结果显示:(1)10个ISSR引物共检测到的位点数在2~13之间,各位点的DNA片段长度介于150~2 000 bp之间。(2)共检测到79个位点,平均每个ISSR引物检测到9.9个位点。(3)多态性条带为65条,多态性位点百分率为42.63%。(4)各种源在分子层面存在明显差异,而这一差异与地理分布呈正相关。(5)小兴安岭林区红松群落的遗传丰富度较高,这也有效的保证了小兴安岭红松群落的林分质量。

Pinus koraiensis is the main tree species in Xiaoxingtinling forest, and of important ecological status in the overall eco-logical system of Xiaoxingtinling area. In order to ensure the genetic richness of Pinus koraiensis in Xiaoxingtinling forest region, and provide high quality Pinus koraiensis afforestation seedlings for Xiaoxing’anling forest area, 10 primers were screened and 10 different Korean pine natural provenances were analyzed by ISSR in this study. The results showed that the points were detected by 10 ISSR primers were between 2 ~ 13loci, DNA fragment length of each point was between 150 ~ 2000 bp. 79 loci were detected, and 9. 9 loci was detected by ISSR primers on the average. Polymorphic bands for the 65 polymorphic loci was 42. 63%. There was a significant difference in the molecular level of the various sources, and this difference was positively correlated to the geographical distribution. The genetic richness of Pinus koraiensis community was higher in Xiaoxingtinling forest area, which effectively ensured the quality ofPinus koraiensis community in Xiaoxingtinling.