摘要
以鼠李糖乳杆菌UV3-4为出发菌株,进行鼠李糖乳杆菌转化亚油酸生产共轭亚油酸转化条件的优化研究。通过单因素试验考察发酵温度、发酵时间、发酵pH和底物亚油酸(LA)添加量对CLA产量的影响,采用响应面法建立CLA产量与各影响因子之间的二次回归方程模型,获得最佳转化条件:发酵温度38℃,发酵时间23.4 h,pH 6.5,底物LA添加量为0.76 mg/m L,此条件下CLA的产量为37.28μg/m L。
This study aims at conditions optimization of the transformation of linoleic acid to conjuga- ted linoleic acid by Lactobacillus rhamnosus UV3-4. The effects of fermentation temperature, fer- mentation time, fermentation pH and LA concentration on the yield of CLA were investigated by sin- gle factor test. Response surface method was used to establish the model of the two regression equa- tions between CLA yield and influencing factors. The optimum conditions were as follows: fermenta- tion temperature 38 ℃, fermentation time 23.4 h, pH 6.5, and addition amount LA 0. 76 mg/mL. Under the above conditions, the yield of CLA was 37.28 μg/mL.
作者
方丽
张芷睿
王鹏
王祖浩
闵伟红
FANG Li ZHANG Zhirui WANG Peng WANG Zuhao MIN Weihong(College of Food Science and Engineering, Jilin Agricultural University, Changchun 130118, China)
出处
《吉林农业大学学报》
CAS
CSCD
北大核心
2016年第6期754-758,765,共6页
Journal of Jilin Agricultural University
基金
吉林省教育厅"十二五"科学技术研究项目[吉教科合字(2012)第57号]
关键词
共轭亚油酸
鼠李糖乳杆菌
响应面法
转化条件
conjugated linoleic acid
Lactobacillus rhamnosus
response surface method
transfor mation condition