响应面法优化鼠李糖乳杆菌产共轭亚油酸的转化条件

Optimization of Transformation Conditions for Producing Conjugated Linoleic Acid in Lactobacillus rhamnosus by Response Surface Method

以鼠李糖乳杆菌UV3-4为出发菌株,进行鼠李糖乳杆菌转化亚油酸生产共轭亚油酸转化条件的优化研究。通过单因素试验考察发酵温度、发酵时间、发酵pH和底物亚油酸(LA)添加量对CLA产量的影响,采用响应面法建立CLA产量与各影响因子之间的二次回归方程模型,获得最佳转化条件:发酵温度38℃,发酵时间23.4 h,pH 6.5,底物LA添加量为0.76 mg/m L,此条件下CLA的产量为37.28μg/m L。

This study aims at conditions optimization of the transformation of linoleic acid to conjuga- ted linoleic acid by Lactobacillus rhamnosus UV3-4. The effects of fermentation temperature, fer- mentation time, fermentation pH and LA concentration on the yield of CLA were investigated by sin- gle factor test. Response surface method was used to establish the model of the two regression equa- tions between CLA yield and influencing factors. The optimum conditions were as follows： fermenta- tion temperature 38 ℃, fermentation time 23.4 h, pH 6.5, and addition amount LA 0. 76 mg/mL. Under the above conditions, the yield of CLA was 37.28 μg/mL.