胰岛素样生长因子-1在大鼠重症急性胰腺炎中的作用

Effects of insulin-like growth factor-1 in the treatment of severe acute pancreatitis

目的观察胰岛素样生长因-1(IGF-1)在重症急性胰腺炎(SAP)大鼠中的治疗作用。方法将30只SD大鼠按随机数字表法分为正常对照组、假手术组、SAP组、高剂量IGF-1组和低剂量IGF-1组,每组6只。采用改良的Aho法制作SAP模型,在造模6 h后处死大鼠,收集胰腺组织、外周血和腹水。采用酶联免疫吸附试验(ELISA)检测各组大鼠血清细胞因子(IL-1β、IL-6)表达。Real-time PCR方法检测胰腺组织(TLR-4、MAPK p38、NF-κB p65)m RNA转录水平及蛋白水平变化。结果 SAP组大鼠腹水量、转氨酶、血清及腹水淀粉酶和炎症细胞因子IL-1β、IL-6较正常对照组及假手术组显著升高,差异均有统计学意义(P<0.05);经IGF-1干预后,大鼠腹水量、转氨酶、血清及腹水淀粉酶、IL-1β和IL-6较SAP组明显下降,且随IGF-1剂量的增加,大鼠腹水量、转氨酶、血清及腹水淀粉酶、IL-1β和IL-6下降更加显著,差异均有统计学意义(P<0.05)。结论 IGF-1能有效减轻SAP大鼠胰腺炎的病情。

Objective To observe the effects of insulin-like growth factor-1 （IGF-1） in the treatment of severe acute pancreatitis （SAP）. Methods Thirty SD rats were randomly divided into five equal groups （n=6 each）： normal group, sham operation group, SAP group, high dose IGF- 1 group and low dose IGF- 1 group. The SAP model was estab- lished by the modified Aho method. Rats were sacrificed 6 h after modeling for collection of pancreatic tissues, peripher- al blood and ascites. The expression status of the serum cytokines （IL-1/3, IL-6） was detected by enzyme-linked immu- nosorbent assay （ELISA）. Real-time PCR was used to detect the changes in TLR-4, MAPK p38 and NF-KB p65 mRNA and protein levels in pancreatic tissues. Results In the SAP group, there were significant increases in ascites volume, transaminases, serum and ascites amylase, IL-1/3 and IL-6, compared with those in the normal group and control group （P〈0.05）. IGF-1 intervention resulted in significant decreases in ascites volume, transaminases, serum and ascites amy- lase, IL-1/3and IL-6, compared to those in the SAP group, and there were greater decreases in ascites volume, transami- nases, serum and ascites amylase, IL-1/3 and IL-6 with increasing IGF-1 dose （P〈0.05）. Conclusion IGF-1 could effectively alleviate pancreatitis in SAP rats.