基于InP@ZnS QDs/Dured纳米荧光探针的DNA检测

InP@ZnS QDs / Dured Fluorescent Nanoprobe for The Detection of DNA

利用巯基丙酸包覆的In P@Zn S量子点(QDs)与Dured构建了一种检测DNA的荧光探针。在该探针中,以环境友好型带负电的In P@Zn S量子点为荧光团,与带正电的Dured通过静电结合,构建了In P@Zn S QDs/Dured纳米荧光探针。通过荧光共振能量转移(FRET)机理,量子点荧光被猝灭;当DNA存在时,Dured与DNA的特异性结合使Dured从In P@Zn S QDs表面脱附,FRET过程被打断,In P@Zn S QDs荧光恢复,以荧光"关-开"方式检测DNA。该探针检测DNA的线性范围为2.0~275.0 ng·L-1,检测限为1.0 ng·L-1,并可用于模拟生物生理条件下的DNA检测。

The fluorescent nanoprobe for the detection of DNA was established by utilizing mercapto- propionic acid coated InP@ ZnS QDs and Dured. In this nanoprobe. The InP@ ZnS QDs/Dured flu- orescent nanoprobes were structured by electrostatic interactions between environment friendly, nega- tively charged InP@ZnS quantum dots and positively charged Dured, and then the fluorescence of InP@ZnS QDs/Dured was quenched through fluorescence resonance energy transfer （FRET）. At presence of DNA, DNA and Dured were specific binding, which enable Dured removing from the surface of InP@ ZnS QDs and achieving fluorescence recovery of InP@ ZnS QDs. Thus, the detec- tion of DNA was realized. The linear range of the InP@ ZnS QDs/Dured fluorescent nanoprobes for DNA detection and the detection limit were 2.0 - 275.0 ng·L^-1 and 1.0 ng·L^-1, respectively. The InP@ZnS QDs/Dured fluorescent nanoprobes can be also used in rapid detection of DNA under simulated physiological conditions.