染色质重塑分子富含AT结合域1A(ARID1A)在胃癌及其配对癌旁组织中的表达及临床意义

Expression and clinical significance of ARID1A in gastric cancer and paired adjacent noncancerous tissues

目的研究胃癌及其配对癌旁组织中染色质重塑因子富含AT结合域1A(ARID1A)的表达及临床意义。方法采用免疫组织化学技术检测90例胃癌组织及其配对癌旁组织中ARID1A蛋白的表达情况。采用非参数Mann-Whitney法和Spearman相关系数分析其与临床病理特征的相关性。采用Kaplan-Meier生存曲线、Log-Rank检验以及COX回归模型进行生存预后分析。结果 ARID1A蛋白在癌旁组织和胃癌组织中胞质和胞核均有表达,在胃癌中的胞质和胞核表达均显著低于癌旁组织。ARID1A在癌旁组织中的胞质表达与肿瘤TNM分期呈显著负相关,其在癌旁组织中胞核表达与年龄呈显著正相关、与肿瘤大小呈显著负相关。生存分析显示,癌旁组织中ARID1A蛋白胞质表达与总生存预后有一定相关性。ARID1A在胃癌组织中的胞质表达和胞核表达均与肿瘤临床病理特征和预后无关。结论 ARID1A在胃癌发生的早期事件中可能有重要的作用,可作为胃癌早期诊断的新靶标。

Objective To investigate the expression pattern and clinical significance of chromatin remodeling AT-rich interactive domain 1A（ ARID1A） protein in gastric cancer and paired adjacent noncancerous tissue samples. Methods The expression of ARID1 A protein was examined in 90 gastric cancer tissue samples and 90 paired adjacent noncancerous tissue samples by immunohistochemistry. The relationship of ARID1 A protein expression with clinical-pathological characteristics was evaluated by Mann-Whitney test and Spearman correlation analysis. The relationship with clinical prognosis was analyzed by Kaplan-Meier survival curve,Log-Rank test and Cox regression model. Results ARID1 A was expressed in both cytoplasm and nucleus of adjacent noncancerous tissues and gastric cancer tissues. Both cytoplasmic and nuclear ARID1 A expressions significantly decreased in gastric cancer tissues compared with adjacent noncancerous tissues. Cytoplasmic ARID1 A expression in adjacent noncancerous tissues was negatively correlated with clinical TNM stage. Meanwhile,nuclear ARID1 A expression in adjacent noncancerous tissues was positively correlated with patients＇ age and negatively correlated with tumor size. Survival analysis showed that cytoplasmic ARID1 A expression in adjacent noncancerous tissues was related with overall survival but not significantly,and that both cytoplasmic and nuclear ARID1 A expressions in gastric cancer tissues were not related with clinical-pathological characteristics and prognosis. Conclusion ARID1 A may play an important role in the early events of gastric carcinogenesis,and it can serve as a potential biomarker for early diagnosis.