诺如病毒GII.17和GI.3基因型引起急性胃肠炎疫情的病原分析

Pathogenic analysis of an acute gastroenteritis caused by norovirus GII.17 and GI.3 genotypes

目的对安庆市某中学暴发的急性胃肠炎疫情进行病原鉴定,并进一步对部分基因序列测序分析,以确定其基因亚型。方法对采集的13份肛拭子,3份生活饮用水标本,采用实时荧光定量RT-PCR方法进行诺如病毒GI/GII型检测,对阳性标本的病毒VP1区部分核酸序列测序和基因库数据比对,并采用MAGA5.0软件构建进化树。结果在13份肛拭子样本7份诺如病毒阳性中,6份属于GI.3型,1份属于GII.17型;3份生活饮用水样本GI.3和GII.17型均有检出。结论该起疫情由诺如病毒感染引起,为GI.3和GII.17基因亚型。

Objective To identify the pathogen outbreaks of acute gastroenteritis in a middle school of Anqing city, and further sequencing of partial gene sequences was used to determine the gene subtype. Methods 13 anal swabs and 3 drinking water samples were detected by fluorescence quantitative PCR method for detection of norovirus type GⅠ/GⅡ. The sequence of positive strains nucleic acid VP1 region were amplified, sequenced and compared with the gene database, and the phylogenetic tree was constructed by MA- GAS. 0 software. Results Ⅰn the 13 samples, 6 samples belonged to type GⅠ. 3, 7 belonged to GⅡ. 17 type. 3 copies of drinking water samples were detected by GⅠ. 3 and GⅡ. 17. Conclusion The epidemic was caused by norovirus GⅠ. 3 and GⅡ. 17 genotypes.