氟对家蚕血液及中肠磷酸酶活性的影响

Effects of Fluoride on Activities of Phosphatase in Hemolymph and Midgut Tissues of Silkworm

运用人工饲料中定量添加氟化物的方法,探讨了氟对家蚕中肠碱性磷酸酶(AKP)、血液酸性磷酸酶(ACP)活性的影响。结果表明,氟对家蚕中肠AKP、血液ACP的活性有明显的抑制作用,并随着添氟浓度的增加而增强,间隔添氟、停止添氟不能减轻这种抑制作用。添食石灰能减轻氟对中肠AKP、血液ACP活性的影响。

Activities of alkaline phosphatase (AKP) in midgut tissue and acid phosphatase (ACP) in hemolymph, respectively, of silkworm (Bombyx mori) were evaluated by adding NaF in artificial diets. Excessive ingestion of fluoride could inhibit activities of ACP in the hemolymph and AKP in the midgut tissue, respectively. Oral administration of different dosages of fluoride exhibited different effects on ACP in the hemolymph and that in the midgut tissues of silkworm larvae. After fluoride poisoning silkworm larvae were fed with diets containing no-fluoride, neither the activity of ACP in the hemolymph, nor that of AKP in the midgut tissues could be recovered. Interval oral administration of fluoride to the larvae had not evident attenuation on ACP in the hemolymph and AKP in the midgut tissues of fluoride poisoning silkworm larvae. The oral administration of Ca(OH)2 to the larvae had evident attenuation on ACP in the hemolymph and AKP in the midgut tissues of fluoride poisoning silkworm larvae, suggesting that Ca2+ could relieve partly toxicity fluorine to silkworm.