室内质控和室间质评在微生物检验中的应用探讨

The internal quality control and external quality assessment in application in microbiological test

目的 :对临床微生物检验过程中,室内质控以及室间质评所发挥的作用进行有效的分析评估,进一步确定临床微生物检验过程中出现的问题。方法 :选取临床检验室间质评200株标本细菌作为主要的研究对象,依照相关的标准分析检验上述200株细菌标本,通过相关的药敏试验完成正确菌株的鉴定。严格按照相关的临床标本的鉴定标准完成鉴定结果跟标本的有效比较及分析,对那些鉴定失误的原因进行深入探讨。结果 :上述所有的细菌均完成转种工作,188株鉴定正确,12株鉴定错误,错误鉴定的是6株革兰阳性杆菌跟6株革兰阴性杆菌。鉴定正确的188株细菌,通过药敏试验,有184例符合,4例不符合,试验结果不符合的抗菌素都是青霉素。结论 :在有效的降低实验室细菌鉴定失误的过程中,临床微生物室间质评发挥着非常重要的作用,所以需要在严格室内质控的基础上,进行微生物检验以及室间质评工作。

Objective Clinical microbiological testing process,internal quality control and the role played by external quality assessment of effective analysis and evaluation,and further determine the clinical microbiological testing process problems.Methods Selected clinical testing EQA 200 bacterial species as the main object of study,in accordance with the relevant standard analytical testing above 200 concurrent bacterial specimens,it further strains identified by related susceptibility testing is completed correctly identified strains above 200 strains complete identification.Strict complete identification results with a valid comparison and analysis of specimens in accordance with accreditation standards related to clinical specimens,identifying the reasons for those failures in depth.Results：All of the bacteria turn the above kinds of work are completed,188 identification is correct,the rest of the 12 identified error,the error is identified six Gram-positive bacilli with six gram-negative bacilli. 188 right above identified bacteria susceptibility testing following results obtained by the test results meet the 184 cases,the test results do not meet there are four bacteria,said the test results are not in line with the antibiotic penicillin.Conclusion：In effectively reduce laboratory errors in the process of identification of bacteria,clinical microbiology EQA plays a very important role,it is necessary to strictly internal quality control on the basis of clinical need for frequent microbiological testing and EQA work.