基于光学相干检测的非接触全光学光声显微镜

Noncontact All-optical Photoacoustic Microscopy Based on Optical Coherent Detection

报道了一种非接触、宽频带、联合微型激光器和低相干迈克尔逊干涉仪的全光学光声显微镜(BD-AOPAM)、光学相干层析系统(OCT)的硬件用于光声信号的检测。目前全光学光声显微镜可检测到的带宽为67 MHz,用碳纤维测得系统的横向分辨率可以达到10.8μm。进一步的,利用包埋头发丝的模拟样品和在体小鼠耳朵血管来验证系统的成像能力。实验结果表明,这种全光学光声显微镜可以在体的实现组织高分辨率的成像,有望成为一种便携式非接触的光声显微镜应用于生物医学当中。

A non-contact broadband all-optical photoacoustic microscopy（ BD-AO-PAM） with a microchip laser and an all-fiber low coherence interferometer was reported. Optical coherence tomography（ OCT） hardware is utilized as the acoustic detector. Currently,the available detection bandwidth of the BD-AO-PAM is 67 MHz,and the lateral resolution measured by carbon fibers reaches 10. 8 μm. Furthermore,the novel system is experimentally verified by the imaging of a scattering phantom embedded with hairs and in vivo vasculature of a mouse ear. The experimental results demonstrate that the BD-AO-PAM can image the tissues with high spatial resolution in vivo,which can be used as a portable non-contact PAM for biomedical applications.