红景天苷对C6胶质瘤细胞系迁移及生长的影响

Effects of salidroside on migration and growth of C6 glioma cell line

目的:研究红景天苷(salidroside,SLDS)对C6胶质瘤细胞系迁移和生长的影响。方法:通过CCK-8实验和LDH实验确定SLDS作用于C6胶质瘤细胞的最适浓度;通过细胞生长曲线、细胞形态图和划痕实验观察SLDS对C6胶质瘤细胞系生长和迁移的影响;通过Western印迹和qPCR检测C6胶质瘤细胞中MMP-9和周期蛋白A(Cyclin A)的表达水平。结果:CCK-8实验和LDH实验显示在12.5,25,50,100和200μmol/L这5个浓度中,100μmol/L的SLDS对C6胶质瘤细胞的抑制最为显著;细胞形态图和生长曲线显示,加入SLDS后C6胶质瘤细胞的生长被显著抑制;划痕实验显示SLDS组肿瘤细胞侵袭力较对照组明显减弱;Western印迹和qPCR显示SLDS组MMP-9和Cyclin A的表达水平明显低于对照组。结论:SLDS对C6胶质瘤细胞的生长及迁移具有抑制作用。

Objective： To investigate the effects of Salidroside on migration and growth of C6 glioma cell line. Methods： CCK-8 assay and LDH assay were applied to determine the optimal effect concentration of salidroside on C6 glioma cells. Cell growth curve, Cell morphology and Wound scratch assay were carried out to illustrate the influence of salidroside on migration and growth of C6 glioma cells. The expression levels of MMP-9 and Cyclin A in C6 glioma cells were tested by Western blot and qPCR. Results： CCK-8 assay and LDH assay demonstrated that within the scope of 12.5, 25, 50, 100 and 200 μmol/L, 100 μmol/L is the optimal effect concentration of salidroside on C6 glioma cells. Cell growth curve and Cell morphology showed that the growth of C6 glioma cells was suppressed obviously after treated with salidroside. Wound scratch assay revealed that the invasiveness of C6 glioma cells had been attenuated in the SLDS group, and Western blot and qPCR demonstrated that the expression levels of MMP-9 and Cyclin A in the SLDS group were evidently lower than the control group. Conclusion： Salidroside inhibits the growth and migration of C6 glioma cell line.