摘要
目的:研究利胆汤对急性重症胆管炎的大鼠所致肝损伤的作用机制。方法:选择健康的Wistar大鼠60只,3月龄,雄性,随机分组为对照组、模型组、胆宁片组、利胆汤低剂量组、中剂量组和高剂量组6组,每组10只。分别于术后立刻、术后6 h、术后12 h、术后24 h 4个时间点收集大鼠胆汁,以及检测大鼠胆总管压力;对照组和模型组灌胃给予0.9%的氯化钠;胆宁片组灌胃给予胆宁片0.5 g/(kg·d);各利胆汤剂量组:6,12和24 g/(kg·d),灌胃给药3天后处死大鼠。HE染色观察大鼠肝病理形态;ELISA检测的方法大鼠血清中的细胞因子IL-6,TNF-α水平;Q-PCR法检测大鼠肝脏巨噬细胞移动抑制因子(migration inhibitory factor,MIF)和Toll样受体4(Toll-like receptor 4,TLR4)基因的表达。结果:利胆汤能明显的降低血清细胞因子IL-6(P<0.05)、TNF-α的表达(P<0.01),并且明显抑制MIF和TLR4的表达(P<0.01)。结论:利胆汤能够改善大鼠急性重症胆管炎所致的肝损伤,作用机制可能通过抑制MIF和TLR4的表达和下调IL-6,TNF-α的表达水平实现的。
Objective: To investigate the mechanism of lidantang for treatment of hepatic injury induced by severe acute cholangitis. Methods: Sixty male Wistar rats were randomly divided into Control group (0.9% NaCl), Model group (0.9% NaCl), Danningpian group (0.5 g/kg), and lidantang group [6, 12, 24 g/(kg·d)]. At 0, 6, 12, 24 h post-surgery, the rat bile was collected, and the pressure of duct were measured. After 3 days of intragastric administration and all rats were killed, pathological morphologic change were observed by HE staining, ELISA was used to assess the levels of serum cytokines IL-6, TNF-α, macrophage migration inhibitory factor (MIF) and Toll-like receptor 4 (TLR4) was assessed by Q-PCR. Results: Lidantang could decrease the level of serum TNF-α (P〈0.01) and reduce the level of serum IL-6 (P〈0.05), moreover, lidantang could also decrease the expression of MIF (P〈0.01) and TLR4 (P〈0.01). Conclusion: Lidantang can attenuate severe acute cholangitis induced hepatic injury via down-regulate the serum of IL-6, TNF-α and inhibit the expression of MIF, TLR4 in the liver.
作者
王建
陈卫东
刘兴洲
吴永丰
WANG Jian CHEN Weidong LIU Xingzhou WU Yongfeng(Zhongda Hospital, Southeast University (Jiangbei), Nanjing 210009, China)
出处
《临床与病理杂志》
2017年第2期353-358,共6页
Journal of Clinical and Pathological Research