摘要
目的观察20月龄以上老龄大鼠急性心肌梗死时,谷氨酰胺对肠屏障功能的损伤后的保护作用。方法将老龄大鼠随机分为非手术组、假手术组、心肌梗死组、谷氨酰胺组。各组大鼠均在模型制作完成后24小时处死,分别测定大鼠血浆中二胺氧化酶(diamine oxidase,DAO)活性和D-乳酸的浓度。在距回盲部5cm处向近端方向切取小肠组织行H&E染色,并对肠粘膜进行病理评分、结果大鼠血浆DAO活性、D-乳酸浓度和肠粘膜评分心肌梗死组最高,其次是心肌梗死—谷氨酰胺组、假手术组、非手术组。谷氨酰胺组DAO活性、D-乳酸浓度和肠粘膜评分与心肌梗塞组比较,具有统计学意义(P<0.05)。结论老龄大鼠心肌梗死时,肠屏障功能严重受损,而谷氨酰胺能减轻肠屏障功能的损伤。
Objective To investigate the protective effects on damaged intestinal barrier function after acute myocardial infarction in senile rats. Methods Senile SD rats were randomly divided into non-surgical control group, sham operation group, myocardial infarction group and glutamine-myoeardial infarction groups. All the rats were killed 24 hours later after myocardial infaretion model was conducted successfully. When blood was collected, DAO activity and D-lactate concentration in plasma were measured. The intestinal tissues around the proximal ileoceens about 5cm were ~moved and , stained by H＆E. Besides, the intestinal histopathological score were evaluated. Results In myocardial infarction group, plasma DAO activity, D-lactate concentration and intestinal score of rats were significantly increased compared with those in non-surgical control group and sham operation groups （P〈0.05）, and all values were improved by glutamine pretreatment. Conclusion The intestinal barrier function was seriously damaged when acute myocardial infarction occurred and the glutamine can protect and maintain the intestinal barrier function in senile rats.
出处
《新疆医学》
2017年第1期23-26,共4页
Xinjiang Medical Journal
基金
国家自然科学基金青年项目(81501202)
关键词
急性心肌梗死
肠屏障
谷氨酰胺
Acute myocardial infarction
Intestinal barrier function
Glutamine
