摘要
介绍了一种新的测定土壤反硝化酶的装置,使土壤反硝化酶(硝酸还原酶、亚硝酸还原酶)的测定更加简便、灵敏、准确。对3种不同质地的土壤从影响土壤反硝化酶活性的3种关键因素:培养时间、称样量和真空度方面进行了研究。结果表明,土壤反硝化酶的最佳培养时间为24—27h,称样量与真空度因酶而异。
A simple and improved method for determination of denitrifying enzymes(nitrate reductase and nitrite reductase)in soil.In comparison with the previous meth ods,it is characterized by more simp le,sensitive and accurate.Three affecting factors including incubation period,sample weight an d vacuum have been optimized through selection of three soils.It has been indicated that the optical incubation period time was 24-27hours,but sample weight and vacuum for the test were variable based on e nzames.
出处
《农业环境保护》
CSCD
北大核心
2002年第4期349-351,共3页
Agro-Environmental Protection
基金
国家自然科学基金部分资助(39970151)
