小鼠肝癌原位移植瘤动物模型的建立

A method of establishing orthotopic transplantable hepatocellular carcinoma in mice

目的 建立小鼠肝癌原位移植瘤动物模型.方法 详尽掌握小鼠肝脏的解剖结构,采用注射器分别将含有1×106、5×105个肝癌H22细胞的小鼠腹腔积液50μl经皮腹腔注射到昆明小鼠肝脏部位,两组各12只,建立小鼠肝脏原位移植瘤模型.观察小鼠生长情况,对肝脏及肿瘤转移组织进行病理学检查,统计动物成瘤情况及肿瘤转移情况.结果 采用小鼠肝脏部位直接注射肝癌细胞株H22的方法建立的两组小鼠原位肝癌接种成瘤率均为100%（12/12）.接种小鼠在实验第6天开始陆续出现腹腔积液,第10天所有小鼠均有腹腔积液产生.1×106接种组小鼠平均生存时间（16.17±3.07）d,5×105接种组平均生存时间（18.08±3.34）d.解剖小鼠,部分可见肾、肠、脾、肠系膜淋巴结转移.结论采用直接注射法可成功建立小鼠肝癌原位移植瘤动物模型,可用于抗肝癌药物的药效学研究.

Objective To establish a method for preparing orthotropic transplantable hepatocellular carcinoma in mice. Methods According to the liver detailed anatomical structure of the mouse, 50 μl mouse ascites containing 1 ×106 and 5 ×105 mouse hematoma H22 cells was input to liver in 12 Kunming mice through percutaneous intraperitoneal injection by syringe, respectively, to establish orthotopic transplantable hepatocellular carcinoma model. The growth status of mice was observed, and the pathological changes of liver and tumor metastasis tissues were detected. The tumor formation and metastasis were analyzed. Results The tumor formation rate was 100% （12/12） by direct injection of mouse hematoma H22 cells in 2 groups. The inoculated mice started to appear ascites at the 6th day, and all mice produced ascites at the 10th day. The survival time was （16.17 ±3.07） d and （18.08 ±3.34） d in 1 ×106 group and 5 ×105 group, respectively. Some mice emerged tumor metastasis in kidney, intestine, spleen and mesenteric lymph nodes. Conclusion The method of direct injection could establish orthotropic transplantable hepatocellular carcinoma model in mice, which can be used for antitumor drug research.