甲状腺乳头状癌中Slug、BRAF V600E及STIP1蛋白的表达及其与被膜侵犯和区域淋巴结转移的关系

Expressions of Slug, BRAF V600E and STIP1 proteins and their correlation with capsular invasion and regional lymph node metastasis in papillary thyroid carcinoma

目的观察甲状腺乳头状癌（PTC）中Slug、BRAF V600E及STIP1蛋白的表达,分析其与被膜侵犯及区域淋巴结转移的关系.方法采用瑞士罗氏Ventana自动免疫组织化学仪检测107例分化型PTC组织中Slug、BRAF V600E及STIP1蛋白的表达,分析其与患者临床病理特征间的关系.结果PTC组织中Slug、BRAF V600E、STIP1蛋白的阳性表达率分别为65.4%（70/107）、61.7%（66/107）、66.4%（71/107）.甲状腺被膜侵犯、中央区淋巴结转移患者Slug、BRAF V600E及STIP1蛋白的阳性表达率高（均P〈0.05）.PTC组织中Slug与BRAF V600E蛋白表达相关（r=0.235,P〈0.05）.结论PTC组织中Slug、BRAF V600E及STIP1蛋白高表达与甲状腺被膜侵犯和中央区淋巴结转移相关,对其进行检测可能有助于预测淋巴结转移及评估预后.

Objective To observe the expressions of Slug, BRAF V600E and STIP1 proteins in papillary thyroid carcinoma （PTC）, and to explore their correlation with capsular invasion and regional lymph node metastasis. Methods Slug, BRAF V600E and STIP1 expressions in 107 cases of differentiated PTC were examined by immunohistochemical staining. The expressions of three proteins and clinicopathological data were statistically analyzed. Results Positive rates of Slug, BRAF V600E and STIP1 in PTC were 65.4 % （70/107）, 61.7 % （66/107） and 66.4 % （71/107）, respectively, and overexpression of Slug, BRAF V600E and STIP1 was significantly associated with capsular invasion and regional lymph node metastasis in PTC （P〈 0.05）. There are a significant correlation between expression of Slug and BRAF V600E in PTC （r= 0.235, P〈 0.05）. Conclusion Overexpression of Slug, BRAF V600E and STIP1 proteins is associated with capsular invasion and regional lymph node metastasis in PTC, which maybe useful for predicting regional lymph node metastasis and prognostic evaluation.