RegIV在视网膜母细胞瘤中的表达及其与血管新生的相关性分析

Expressions of RegIV in Retinoblastoma and Its Correlation with Angiogenesis

目的:研究RegIV(再生基因Ⅳ)在视网膜母细胞瘤中的表达及其与血管新生的相关性。方法:选择2012年6月-2014年6月在我院行眼球摘除术治疗的视网膜母细胞瘤患儿16例为研究对象。采用免疫组化染色法对16例视网膜母细胞瘤患者肿瘤组织及16例正常视网膜组织标本中的RegIV、VEGF-A(血管内皮生长因子-A)、VEGF-C(血管内皮生长因子-C)的表达进行检测,并分析RegIV的表达与视网膜母细胞瘤患者的临床病理特征及VEGF-A、VEGF-C表达的相关性,同时比较RegⅣ阳性表达病例肿瘤组织与正常视网膜组织中微血管密度(MVD)之间的差异。结果:视网膜母细胞瘤组织中RegⅣ、VEGF-A、VEGF-C的表达水平均较正常视网膜组织显著上升,差异有统计学意义(P<0.05)。视网膜母细胞瘤患者瘤组织中RegⅣ、VEGF-A、VEGF-C表达均与其临床分期、病理分型及视神经侵犯与否均显著相关(P<0.05),与性别、发病部位无关(P>0.05)。视网膜母细胞瘤组织中RegⅣ的表达与VEGF-A表达呈正相关(r=0.696,P<0.05),与VEGF-C表达呈正相关(r=0.728,P<0.05)。视网膜母细胞瘤组织中RegⅣ阳性表达的病例MVD值为(59.67±1.43),明显高于正常视网膜组织中MVD值(30.53±2.31)(P<0.05);视网膜母细胞瘤组织中RegⅣ阴性表达病例MVD值为(38.04±2.86),与正常视网膜组织中MVD值比较无明显差异(P>0.05)。结论:RegIV在视网膜母细胞瘤中呈高表达,且与肿瘤的侵袭与转移相关,其可能通过促进VEGF-A、VEGF-C表达促进血管新生,进而加速视网膜母细胞瘤的发生和进展。

Objective： To study the expression of regenerating islet-derived family member 4（RegIV） in retinoblastoma and its correlation with angiogenesis. Methods： 16 cases of patients with retinoblastoma who underwent operation in our hospital from June2012 to June 2014 were selected as the research object. The positive expression rates of RegIV, VEGF-A and VEGF-C between retinoblastoma tissues and normal tissues were compared, the correlation of clinicopathological features with the expressions of RegIV,VEGF-A and VEGF-C were analyzed, and theh microvessel density（MVD） between RegIV positive expression tumor tissue and normal tissue were compared. Results： Compared with the normal tissues, the RegIV, VEGF-A and VEGF-C expressions were higher in the retinoblastoma tissues（P〈0.05）. The RegIV, VEGF-A, VEGF-C expressions were significantly correlated with the clinical stage, pathological type, optic nerve invasion retinoblastoma（P〈0.05）, but had nothing to do with gender, site of onset（P〈0.05）. Pearson correlation analysis showed the expression of RegIV was positively correlated with VEGF-A（r=0.696, P〈0.05）; the expression of RegIV was positively correlated with VEGF-C（r=0.728, P〈0.05）. The MVD value of RegIV positive expression was（59.67±1.43）, which was significantly higher than that of the normal retinal tissues（30.53±2.31）（P〈0.05）; the MVD value of RegIV negative expression tissues was（38.04±2.86）, which had no difference compared with the normal retinal tissues（P〈0.05）. Conclusions： The positive expression rates of RegIV was highly expressed in the retinoblastoma tissue and correlated with the invasion and migration, it could accelerate the development of the retina through promoting the angiogenesis mediated by VEGF-A and VEGF-C.