TLR9介导的Myd88信号通路分子在稽留流产患者绒毛组织中的表达及其临床意义

Expression and Clinical Significance of TLR9 Mediated Myd88 Signaling Pathway in the Patients with Missed Abortion

目的:研究TLR9介导的Myd88信号通路在稽留流产患者绒毛组织中的表达及其临床意义。方法:本研究收集了稽留流产患者及正常分娩者的绒毛组织,采用Real-time-PCR及Western-blot方法检测了TLR9介导Myd88信号通路中TLR9、Myd88、TNF-a、NF-κB、IRF1 mRNA和蛋白表达。结果:与正常对照组相比较,TLR9、Myd88、TNF-a、NF-κB、IRF1 mRNA和蛋白在稽留流产组患者绒毛组织中相对表达水平显著升高,差异有统计学意义(P<0.05)。结论:稽留流产的发生与TLR9/Myd88信号通路有关。

Objective： To study the expression and clinical significance.of TLR9 mediated Myd88 signaling pathway in patients with missed abortion. Methods： The villi tissue of missed abortion patients and normal delivery were collected. Real- time PCR and Western blot method were used to detect the mRNA and protein expression of TLR9, Myd88, TNF-a, NF-κB, IRF1. Results： Compared with normal control group, the mRNA and protein expression of TLR9, Myd88, TNF-a, NF-κB, IRF1 were significantly raised in the abortion group（P〈0.05）. Conclusion： TLR9/MyD88 signaling pathway might be involved in the pathogenesis of missed abortion.