白癜风患儿抗核抗体、免疫球蛋白及补体检测的临床分析

Clinical Analysis of Antinuclear Antibodies,Immunoglobulin and Complement in Children Patients with Vitiligo

目的:探讨白癜风患儿中抗核抗体(ANA)、免疫球蛋白(IgG、IgA、IgM)以及补体(C3、C4)的表达及临床意义。方法:收集2014年10月至2016年5月我院收治的30例白癜风患儿为病例组,并于同期随机选取30例健康体检儿童为对照组,患儿的血清ANA、12种自身抗体谱分别采用间接免疫荧光法及免疫印迹法进行检测,IgG、IgA、IgM,C3、C4水平采用免疫透射比浊法进行检测。结果:病例组中ANA阳性率为13.33%(4/30),与对照组的3.33%(1/30)比较,差异无统计学意义(P>0.05)。12种自身抗体谱中,分别有1例(3.33%)患儿dsDNA、SmDNA、SS-A/Ro60KD、SS-B/La、CENP-B阳性,与对照组比较,差异均无统计学意义(P>0.05)。病例组患儿血清IgG、IgA、C4水平低于对照组,差异有统计学意义(P<0.05)。ANA阳性患儿血清IgG、IgA、IgM水平高于ANA阴性患儿,而补体C3、C4水平低于ANA阴性患儿,差异有统计学意义(P<0.05)。结论:白癜风患儿ANA阳性表达与健康儿童无明显区别,体液免疫功能有明显异常,临床有重要的参考价值。

Objective： To investigate the expression and clinical significance of anti nuclear antibody（ANA）, immunoglobulin（IgG, IgA, IgM） and complement（C3, C4） in patients with vitiligo. Methods： 30 children patients with viligo in our hospital from October to May 2016 as case group were collected, and at the same time, 30 healthy children as control group were randomly selected. The serum ANA and 12 kinds of autoantibodies were detected by indirect immunofluorescence and Western blotting respectively, IgG, IgA, IgM, C3 and C4 levels were detected by immune transmission turbidimetry method. Results： Compared with of 3.33%（1/30） in control group,the positive rate of ANA in case group was 13.33%（4/30）, and the difference was not statistically significant（P〉0.05）. In the 12 kinds of antoantibodies spectrum, there was 1 case patient（3.33%） of positive rate about dsDNA, SmDNA, SS-A/Ro60 KD, SS-B/La, CENP-B respectively, which was no obviously difference compared to those in control group（P〉0.05）. The serum IgG, IgA and C4 levels were lower than that in control group, the difference was statistically significant（P〈0.05）. The IgG, IgA, IgM levels of children patients of ANA-positive were higher than those of ANA-negative patients, the difference was statistically significant（P〈0.05）. The C3, C4 levels of complement of children patients of ANA-positive were lower than those of ANA-negative patients, the difference was statistically significant（P〈0.05）. Conclusion： There is no obviously difference on ANA-positive expression between children patient with vitiligo and healthy children while the humoral immune function has obvious abnormal, it has an important reference value in clinic.