直肠癌组织中PTEN和Notch1的表达及其与临床病理参数的关系

The Expression of PTEN and Notch1 in Rectal Cancer and Their Relationship with Clinical Pathological Parameters

目的:探究直肠癌组织中PTEN和Notch1的表达及其与临床病理参数的关系。方法:收集2014年1月~2015年4月,我院肿瘤外科确诊48例直肠癌病理组织标本与20例直肠癌患者癌旁正常组织标本为研究对象,应用免疫组化链霉菌抗生物素蛋白-过氧化物酶连结法(S-P法)分析PTEN和Notch1蛋白在直肠癌病理组织和正常组织中的表达情况。结果:PTEN和Notch1主要表达于直肠癌组织细胞质中,呈现棕黄色;Notch1在直肠癌病理组织中表达率显著高于正常组织(P<0.05);PTEN在正常组织表达率显著高于在直肠癌组织(P<0.05);Notch1在淋巴转移、中底分化及分期为C期患者病理组织中表达率较高(P<0.05);PTEN在无淋巴转移、中底分化程度及A+B期患者病理组织表达率较高(P<0.05);相关性分析显示,Notch1和PTEN在直肠癌病理组织中表达呈现负相关关系(r=-0.534,P=0.000)。结论:Notch1和PTEN表达失调在直肠癌的发生、发展和淋巴结转移中有重要作用;PTEN表达可能对直肠癌的发生、发展具有抑制作用,Notch1表达可能对直肠癌的发生、发展具有促进作用。

Objective： To explore the expression of PTEN and Notch1 in rectal cancer and their relationship with clinical pathological parameters. Methods： 48 cases of pathological tissues and 20 cases of carcinoma adjacent tissue were collected in oncology department of our hospital from January 2014 to April 2015 as research objects; Immunohistochemical method of the anti biotin peroxidase connection of streptomyces（S-P） was applied to analyse the expression of PTEN and Notch1 protein in colorectal cancer tissues and normal tissues. Results： PTEN and Notch1 were mainly expressed in the cytoplasm of the cells of rectal cancer, showed a brown yellow; The expression rate of Notch1 in colorectal cancer was significantly higher than that in normal tissues（P〈0.05）; The expression rate of PTEN in normal tissues was significantly higher than that in colorectal cancer tissues（P〈0.05）; The higher expression rate of Notch1 in lymph node metastasis, middle and bottom differentiation and C stage patients was higher（P〈0.05）; The expression rate of PTEN was higher in the patients with no lymph node metastasis, middle and bottom differentiation and A＋B stage（P〈0.05）;Correlation analysis showed that the expression of Notch1 and PTEN in colorectal cancer was negatively correlated（r=-0.534, P=0.000）.Conclusion： The dysregulation of PTEN and Notch1 expression may be associated with the occurrence and development of colorectal cancer and lymph node metastasis. PTEN expression has probable inhibitory effect on the occurrence and development of colorectal cancer and Notch1 expression can probably promote the occurrence and development of rectal cancer.