地顶孢霉培养物对奶牛乳中氨基酸和后肠道菌群的影响

Effect of Acremonium terricola culture on the amino acids in milk and hindgut microflora of dairy cows

为了研究地顶孢霉培养物对奶牛乳中氨基酸和后肠道菌群的影响,试验选择30头荷斯坦奶牛,根据产奶量、泌乳天数和胎次随机分为3组,分别饲喂基础日粮、基础日粮+15 g/d地顶孢霉培养物、基础日粮+30 g/d地顶孢霉培养物,预试期7 d,正试期56 d,在正试期的第28,56天收集奶样,测定乳中的氨基酸含量;在正试期的第54,55,56天收集粪样,测定后肠道中大肠杆菌、乳酸菌和双歧杆菌的数量。结果表明:地顶孢霉培养物显著提高了乳中总氨基酸(P<0.05)、必需氨基酸(P=0.02)和风味氨基酸(P<0.05)的含量。与对照组相比,添加地顶孢霉培养物组奶牛后肠道乳酸菌的数量显著提高(P<0.05)。说明地顶孢霉培养物对提高乳质量和改善乳风味有积极影响,并且能够提高奶牛后肠道益生菌数量,且30 g/d的添加量效果最好。

To study the effect of Acremonittm terricola culture on the amino acids in milk and hindgnt microflora of dairy cows, thirty Holstein cows were selected and randomly divided into 3 groups according to milk yield, lactation days and parity. The cows in 3 groups were fed basal diet, basal diet supplemented with 15 and 30 g/d Acremonium terricola culture, respectively. The pre - test period was 7 d, and the test period was 56 d. Milk samples were collected on days 28 and 56 of the test period, and then the contents of amino acids were measured. Fecal samples were collected on days 54, 55 and 56 of the test period, and then the numbers of Escherichia coli, lactic acid bacteria and bifidobacteria from the hindgut were measured. The results showed that Acremonium terricola culture significantly increased the contents of total amino acid （ P 〈 0. 05）, essential amino acids （P =0.02） and flavour amino acids （P 〈0.05） in milk. The numbers of lactic acid bacteria from the hindgnt of dairy cows in the two groups added with Acremonium terricola culture were markedly increased （ P 〈 0.05 ） compared with the control group. The results indicate that Acremonium terricola culture has a positive effect on improving the quality and flavor of milk, and can increase the numbers of probiotics from the hindgut of dairy cows, while the best effect is obtained at the addition level of 30 gild.