菊花品种表型性状与SSR和SCoT分子标记的关联分析

Association Analysis of Phenotypic Traits with SSR and SCoT Molecular Markers in Chrysanthemum morifolium

利用16对SSR和10对SCoT引物对99个菊花品种的基因组变异进行扫描,分析其群体结构,并采用一般线性模型GLM和混合线性模型MLM方法对10个表型性状进行关联分析。供试群体Shannon’s指数为1.46~2.52。群体遗传结构分析将99个菊品种划分为5个亚群。GLM分析发现有8个SSR位点与7个表型性状相关联(P<0.01),其中与3个花部性状(花序直径、花序高度、舌花宽度)相关位点5个,与4个营养性状(叶柄长度、叶片长度、托叶大小及叶一级刻度)相关位点4个,各位点对表型变异的解释率在0.06~0.40之间;3个SCoT位点与花序直径相关联。MLM分析发现3个SSR位点与4个表型性状相关联。MLM中检测到的标记JH42、JH81和JH86在GLM中同样被检测到,但解释率稍低。

Association analysis is a procedure for detecting QTLs as well as their alleles based on linkage disequilibrium. The genotyping data of 16 SSR and 10 SCoT markers on 99 Chrysanthemum morifolium cultivars were used to detect associations with 10 phenotypic traits by using GLM and MLM methods. The Shannon’s index of the population ranged from 1.46–2.52. Genetic structure analysis showed that the selected cultivars were composed of 5 subpopulations. There were 8 SSR loci associated with 7 phenotypic characters（P 〈 0.01）,among which 3 traits of propagative organ（inflorescence diameter,inflorescence height,ligulate flower width）were associated with 5 loci,while 4 traits of vegetation organ（petiole length,leaf blade length,stipule size,lamina magnitude）were associated with 4 loci by using GLM models. The ratio of explanation on the phenotype of individual locus ranged from 0.06–0.40. Inflorescence diameter was significantly associated with three SCoT loci by using GLM models. Four phenotypic characters were associated with 3 SSR loci based on MLM models. These markers（JH42,JH81 and JH86）detected by MLM method can be found according to GLM method similarly. Howerver,the ratio of explanation obtained by MLM method was slightly lower than by GLM method.