黑虎掌菌粗多糖制备及体外抗氧化活性研究

Preparation of polysaccharides from Sarcodon imbricatus and their in vitro antioxidant activity

以云南和四川黑虎掌菌子实体为原料,热水浸提黑虎掌菌子实体粗多糖,并采用响应面法优化提取工艺。结果表明,云南黑虎掌菌粗多糖(YSP)的最佳提取条件为提取时间3.1 h,提取温度91℃,水料比60∶1(mL∶g),多糖得率16.75%;四川黑虎掌菌粗多糖(SSP)的最佳提取条件为提取时间3.1 h,提取温度93℃,水料比58:1(mL:g),多糖得率13.93%。以YSP和SSP为实验样品,VC为阳性对照,羟基自由基、DPPH自由基以及超氧阴离子自由基清除率为检测指标,评价YSP与SSP的体外抗氧化活性。结果表明:SSP的羟基、DPPH及超氧阴离子自由基最高清除率分别为86.14%、71.78%和99.98%,均高于对应的YSP清除率76.54%、58.52%和99.93%,且其超氧阴离子自由基清除率均高于VC,但羟基自由基和DPPH自由基清除率均低于VC。

Ytmnan Sarcodon imbricatus polysaccharides （YSP） and Sichuan S. imbricatus polysaccharides （SSP） were extracted from fi＇uit body of S. imbricatus, and the extraction process was optimized by response surface method. The optimum extraction conditions were confirmed as follows： for YSP： extraction time 3.1 h, temperature 91 ℃, liquid to solid ratio 60：1 （ml：g）, and the polysaccharide yield was 16.75%; For SSP： extraction time 3.1 h, temperature 93 ℃, liquid to solid ratio 58：1 （ml：g）, and the polysaccharides yield was 13.93%. Furthermore, using YSP and SSP as experimen- tal samples, VC as the positive control, the/n vitro antioxidant activities ofpolysaccharides on hydroxyl free radical, DPPH free radical and superoxide anion free radical were investigated. The results showed that the highest scavenging rates of SSP to hydroxyl, DPPH and superoxide anion radicals were 86.14%, 71.78% and 99.98%, respectively, which were higher than that of YSP （76.54%, 58.52% and 99.93%）. The superoxide anion free radical scavenging rate of both polysaccaride were higher than VC, but the DPPH free radical and hydroxyl free radical scavenging rate were lower than VC.