V_E,IGF-I和hCG对体外培养小鼠睾丸间质细胞增殖的影响

Effects of V_E,IGF-I and hCG on Proliferation of Mice Leydig Cells in Vitro

为了探究不同浓度维生素E(V_E)、胰岛素样生长因子(IGF-I)、人绒毛膜促性腺激素(hCG)对睾丸间质细胞(LCs)增殖的作用,试验通过添加不同浓度V_E,IGF-I,h CG分别处理小鼠睾丸间质细胞24,48 h,采用四唑盐比色法(MTT法)检测细胞活力。结果表明,与对照组相比,24 h时V_E组细胞活力显著增加(P<0.05),IGF-I,h CG组差异不显著(P>0.05);48 h时,V_E,h CG组细胞活力差异显著(P<0.05),IGF-I组差异不显著(P>0.05)。说明在24,48 h的IGF-I对细胞的增殖变化没有明显促进作用,V_E和h CG对细胞增殖有一定的促进作用。

To assess the promotive role of V_E, IGF-I, hCG in leydig cells（LCs）proliferation, in this study the activity of LCs exposed to different concentrations of V_E, IGF-I, h CG was detected by using MTT assay. The results showed that 24 h V_Egroup administration significantly increased cell proliferation compared with control group（P〈0.05）, while IGF-I and h CG groups showed no significant effect. After 48 h, cell proliferation in V_Eand h CG groups increased significantly compared with control group（P〈0.05）.However, IGF-I group had no significant change. This study illustrated that IGF-I was not marked improve on cell proliferation at this time slot, V_E, h CG had certain promotion effect on cell proliferation.