摘要
目的将制备的羟基磷灰石/壳聚糖-转化生长因子-β1缓释微球复合涂层种植体运用于糖尿病兔胫骨,探讨其周围Runx-2/ALP/OC基因表达的改变,从分子水平评估该种植体的成骨性。方法制备复合涂层种植体,运用扫描电镜(SEM)分析其表面形貌。并建立SPF级新西兰兔2型糖尿病模型,分别在其胫骨干骺端植入种植体,分别于术后4、8、12周,取出带种植体周围骨组织,采用实时荧光定量PCR(RT-PCR)分别检测普通图和糖尿病兔在不同种植体植入后Runx-2、碱性磷酸酶(ALP)、骨钙素(OC)、晚期糖基化终末产物(AGEs)的表达量。结果 Runx-2、ALP、OC在改性涂层种植体表面的mRNA表达在同一时间点上,改性种植体组的表达量高于普通种植体组;不同时间点上,使用改性种植体的Runx-2、ALP、OC的基因表达量增加高于普通种植体,正常兔改性种植体基因表达最高。结论复合涂层种植体能促进高糖环境下Runx-2/ALP/OC基因表达,具有一定的临床运用前景。
Objective This study investigated the effects of hydroxyapatite（HA）/chitosan（CS）-transforming growth factor-β1（TGF-β1）composite coatings on implant surfaces,as well as on the symbol gene of diabetic rabbit osteoblasts.MethodsCoatings were prepared on implant surfaces and studied by scanning electric microscope（SEM）,New Zealand White rabbits were used to establish the model of type 2diabetes.Implant the implants in tibial metaphysis of the rabbit and took them and surrounding bone tissue our after 4,8,12 weeks.Detcet the expression of AGEs,ALP,OC,Runx-2 mRNA by semi-quantitative RT-PCR.Results The expression level of Runx-2,ALP and OC mRNA on composite coatings implants tended to be higher than that on normal implant at each time point.The same differences occurred at different points in time,especially the implants in normal rabbits.Conclusion The composite coatings implants significantly promote the proliferation of osteoblast and the expression of Runx-2,ALP and OC in high glucose,and it＇s worth of further clinical use.
出处
《重庆医学》
CAS
北大核心
2017年第8期1023-1026,共4页
Chongqing medicine
基金
口腔疾病研究国家重点实验室开放课题(SKLOD2015OF09)
四川省卫生和计划生育委员会科研课题(16PJ173)
关键词
糖尿病
逆转录聚合酶链反应
种植体
表面改性
diabetes mellitus
reverse transcriptase polymerase chain reaction
implant
surface modification
