去铁敏对大鼠蛛网膜下腔出血后脑积水的影响

The efficacy of deferoxamine on hydrocephalus after subarachnoid hemorrhage in rats

目的探索去铁敏对于大鼠蛛网膜下腔出血(SAH)后脑积水的作用。方法雄性SD大鼠随机分为假手术对照组、模型组、给药组。采用二次枕大池注血模型制作蛛网膜下腔出血模型,于术后第1、14、28天进行神经功能评分,处死大鼠后采用HE染色观察侧脑室大小,采用Western Blot和免疫组化方法检测大鼠侧脑室周围脑组织TGF-β、TNF-α、IL-1β的表达情况。结果模型组侧脑室面积显著高于正常对照组,去铁敏治疗组在14天和28天能够显著改善脑积水。SAH后侧脑室周围脑组织TGF-β在第1天和第28天呈双相增高,TNF-α第1天即增高且持续至第28天,IL-1β没有显著变化。去铁敏治疗组与模型组相比,在第1天显著降低TNF-α的表达,在第28天显著降低TGF-β的表达。结论去铁敏治疗能降低大鼠SAH后脑积水的发生率,通过抑制炎症反应及改善蛛网膜纤维化过程发挥作用。

Objective To explore the efficacy of deferoxamine on hydrocephalus after subarachnoid hemorrhage （SAH）. Methods Male Sprague Dawley rats were randomly assigned into 3 groups：sham group,SAH group,and deferoxamine-treated group. All the rats except control group were received twice injecion of autologous blood into cisterna magna. Deferoxamin （100mg/kg）was administered 3 hours after SAH and then every 12 hours up to 7 days in SAH group. Rats were sacrificed at Day 1,14,and 28 for measurement. Haematoxylin Eosin（HE）staining were used to examine the size of the lateral ventricles. Western Blot and immunohistochemical methods were applied to the expression of TGF-β,TNF-α,and IL-1β. Results The size of the lateral ventricles was significant larger in SAH group than that in control group,and deferoxamine therapy could improve hy- drocephalus after SAH at Day 14 and 28. After suffering SAH,TGF-β was increased immediately at Day 1 ,recovered at Day 14,and then again in- creased at Day 28. TNF-a was increased at Day 1 and continued at a high level until the end of the observation. While IL-1β was observed no fluctuation. Deferoxamine could reduce the expression of TNF-α at Day 1 and TGF-β at Day 28. Conclusion Deferoxamine could reduce the inci- dence of the hydrocephalus after SAH. It might inhibite the inflammatory response at early stage and improve arachnoid fibrosis at late stage.