1953-2016年基孔肯雅病毒的系统进化及蛋白质多样性分析

Phyletic evolution and protein polymorphism of chikungunya virus between 1953 to 2016

目的对1953-2016年基孔肯雅病毒的主要流行株进行分子进化特征和氨基酸位点变异情况分析,为防控基孔肯雅病毒的流行提供理论依据。方法在NCBI数据库检索1953-2016年基孔肯雅流行株,按照暴发情况筛选代表性毒株35株,使用Scan Prosite软件对基孔肯雅病毒蛋白的功能区域进行预测,用Clustal X和Mega 6.06生物软件对于筛选的35株代表株的核苷酸和氨基酸序列进行分析,构建种系进化树并分析氨基酸变异情况析。结果进化树显示35株基孔肯雅病毒对应其3个基因型分支为3簇,分别对应基孔肯雅病毒的3个基因型:东/中/南非型(19株)、亚洲型(13株)和西非型(3株),各蛋白同源计分比值均为0.96以上。E2关键抗原表位(共19个氨基酸)也有变异,其中19株东/中/南非型毒株序列有1处位点(H18Q)发生替换,西非型毒株未发生变化,13株亚洲型毒株也显示有1处位点(N5H)变化。结论基孔肯雅病毒进化较为保守,但在其重要功能区域出现一些位点的突变,关注其进化情况对于基孔肯雅防控工作有积极意义。

Objective To analyze the evolutionary characteristics and the amino acid mutation of chikungunya virus（CHIKV）strains between 1953 to 2016,and provide theoretical basis for the prevention and control of CHIKV. Methods Published CHIKV sequences in NCBI was filtered according to their outbreak background;functional region of CHIKV was predicted with Scan Prosite;nucleotide and amino acid sequences of 35 filtered strains was used for conducting the analysis of amino acids and the construction of phylogenic tree was processed with Clustal X and Mega 6.06. Results Phylogenic tree showed 35 CHIKV strains gathered to three genotypes;the BSR value of all the protein was above 0.96. and mutations was observed in the key epitope of E2（19 Amino acids）, a H18 Q mutation was found in 19 east/central/south Africa strains, the BSR value of all the protein was above 0.96. and a N5 H mutation was found in the 13 Asia strains,with no mutation found in the key epitope of E2 of West Africa strains. Conclusions Though the evolution of CHIKV is relatively conservative,some amino acids were mutated. Understanding of its mutation contributes may to the prevention and control of CHIKV.