人乳头瘤病毒感染与结直肠癌的相关性

Association between human papillomavirus infection and colorectal cancer

目的检测195例结直肠癌样本中人乳头瘤病毒(HPV)的感染情况。方法利用通用PCR法检测结直肠癌样本中的HPV DNA,随后用荧光定量PCR和直接测序法对HPV DNA阳性样本进行HPV分型,同时统计HPV阳性标本的Kras、PIK3CA和BRAF基因突变情况和临床病理特征。结果结直肠癌标本中HPV感染阳性率为17.94%(35/195),以HPV16、18型感染为主,且存在混合感染;HPV感染阳性标本中Kras基因突变率为42.85%,PIK3CA、BRAF基因突变率均为2.86%,HPV感染与三个基因突变的相关性均无统计学意义;阳性标本中以右半结肠的癌变居多,癌组织以中分化为主,临床Ⅱ期多见,多无淋巴结的转移。结论 HPV感染可能与结直肠癌的发生有关,但与Kras、PIK3CA、BRAF基因突变无关。

Objective To study the relationship between human papillomavirus（HPV）infection and colorectal carcinoma.Methods HPV DNA in the colorectal carcinoma was amplified by general primer-mediated PCR（GP-PCR）,and HPV types were performed by Real- time PCR and sequence analysis. The relationship between HPV infection and the colorectal carcinoma,Kras,PIK3 CA,and BRAF gene mutations were analyzed. Colon cancer pathologies were also investigated.Results The positive rate of HPV DNA detection was 17.94%（35/195）in the colorectal carcinoma;HPV type 16 and 18 were the most frequently viral type and mixed infection was existed. The mutation rates of Kras gene was 42.85%,and both PIK3 CA and BRAF mutation were 2.86%. There were not significant correlation between HPV infection and these three gene mutations.Among the HPV positive cases,most of the carcinomas were in the right colon,and the clinical stage Ⅱ was more common,with no lymph node metastasis. Conclusion HPV infection could be correlated with colorectal cancer,but might not be related to Kras,PIK3 CA and BRAF mutations.