‘云香’水仙NAC基因克隆及表达分析

Cloning and Expression Analysis of NAC Gene from Narcissus tazetta var. chinesis ‘Yunxiang'

从‘云香’水仙转录组文库中筛选出1条NAC基因序列,采用PCR技术克隆得到其c DNA序列。序列分析表明,该NAC基因包含822 bp的开放阅读框,编码273个氨基酸,分子量30 903.9 u,命名为Nt NAC3153(Gen Bank登录号:KU375569)。理化性质分析和多重序列比对显示,Nt NAC3153蛋白是一个无跨膜区域的亲水性蛋白,在N端具有一段保守结构域。系统进化树分析表明,Nt NAC3153与同为单子叶植物的小果野芭蕉Ma NAC、二穗短柄草Bd NAC和小米Si NAC亲缘关系最近。实时荧光定量PCR分析显示,Nt NAC3153基因能够被ABA、盐胁迫和高温诱导表达。研究表明:Nt NAC3153可能参与‘云香’水仙的抗逆调控机制。

A sequence of one NAC gene from ‘Yunxiang＇ transcriptome library was cloned using PCR technology.Sequence analysis indicates that the c DNA of NAC, named Nt NAC3153（Gen Bank accession number： KU375569）,contained an open reading frame of length 822 bp（ encoding 273 amino acids, molecular weight： 30.903 9 ku）.Physiochemical properties analysis and multiple sequence alignment showed that Nt NAC3153 protein was a hydrophilic protein which was free of trans-membrane region and had a conserved N-terminal domain. Phylogenetic analysis indicated that the Nt NAC3153 had the closest relationship with Musa acuminata Ma NAC, Brachypodium distachyon Bd NAC and Setaria italica Si NAC. Real-time PCR analysis showed that the expression of Nt NAC3153 gene could be induced by ABA, salt stress and high temperatures. Nt NAC3153 may be involved in the regulation of resistance mechanisms of ‘Yunxiang＇.