混合浓缩血小板保存期质量变化研究

Study on the quantity of pooled platelet concentrates during storage period

目的探讨混合浓缩血小板制剂(PC)保存期质量变化。方法对86例(份)(400 m L/份)新鲜全血采用白膜法制备PC,按相同血型汇集,汇集后血小板数>2.7×1011个/治疗量,并经白细胞滤器系统(含混合PC保存袋)过滤,于滤后0、3、5 d观察血小板存活率、p H、PO2、PCO2、GLU、Lac、HSR、聚集率及CD62p表达率。结果 13份混合PC保存0和5 d的血小板计数(×1011个)为2.88±0.28 vs 2.66±0.27(P>0.05),保存5 d的PC血小板存活率达89.46%;0和3 d的GLU(mmol/L)为18.75±0.47 vs 17.52±0.54,Lac(mmol/L)为4.30±0.46 vs 7.59±1.22,CD62p(%)为10.90±5.93 vs 32.74±8.12和AGG(%)为91.38±3.10 vs 52.42±21.68(P<0.01);0和5 d的p H为7.01±0.13 vs 6.90±0.13(P>0.05);3和5 d的AGG(%)为52.42±21.68 vs 36.29±27.46(P>0.05)。结论混合PC保存袋保存的混合PC质量在保存期存在下降趋势,但在保存5 d仍符合国家标准,可以供临床输用。

Objective To explore the quality of pooled platelet concentrates during storage period. Methods In a routine method,platelets of identical blood type were pooled from buffy coat until the total platelet count was up to 2. 7 × 10^11 per therapeutic dose,and then leukocytes were filtrated with the filter within 24 hrs after blood collection. All leuko-reduced pooled platelets were kept at 22 ℃ in centrifuge for 5 days. The following parameters,p H,p O2,PCO2,GLU,Lac,HSR,AGG and CD62 p,were observed in all pooled platelets（ a total number was 13） on d0,d3 and d5. Results Platelets counts（ × 10^11） showed no significant difference at the end of storage（0 d：2. 88 ± 0. 28 vs 5d： 2. 66 ± 0. 27）. The platelet＇s survival rate was 89. 46% on 5 d. Compared to 0 d,GLU（ mmol / L）（ 18. 75 ± 0. 47 vs 17. 52 ± 0. 54）,Lac（ mmol / L）（4. 30 ± 0. 46 vs 7. 59 ± 1. 22）,CD62P（%）（10. 90 ± 5. 93 vs 32. 74 ± 8. 12） and AGG（%）（91. 38 ± 3. 10 vs 52. 42 ±21. 68） were significant different（ P〈0. 01） on 3 d. After 5 days,p H（0 d：7. 01 ± 0. 13 vs 5 d：6. 90 ± 0. 13） and AGG（%）（3 d：52. 42 ± 21. 68 vs 5 d：36. 29 ± 27. 46） declined markedly. Conclusion Pooled platelet concentrates stored in the storage bag with leukocyte filter within 5 days can be used for transfusion.