摘要
采用构建猪源MAVS重组质粒pEGFP-MAVS,将其转染PK-15细胞24h后,再感染口蹄疫病毒(MOI=0.1),通过Western blot和real-time PCR检测MAVS融合蛋白表达及对口蹄疫病毒感染宿主细胞的复制和病毒感染滴度。结果显示:重组质粒pEGFP-MAVS能在PK-15细胞中获得表达,并且上调MAVS基因对口蹄疫病毒在宿主细胞中的早期复制有显著抑制作用,病毒的感染滴度也有一定的降低。结果表明:MAVS具有一定的抗病毒作用,此为进一步研究口蹄疫病毒逃逸宿主天然免疫的机制奠定了基础。
To study the impact of swine mitochondrial antiviral signaling protein MAVS on the in- fection of foot-and-mouth disease virus (FMDV) to host cells,recombinant plasmid swine MAVS pEGFP-MAVS was constructed, after transfecting PK-15 cells 24 h, and then infected FMDV (MOI=0. 1). Western blot and real-time PCR were used to detect expression of MAVS fusion protein and its replication of FMDV infection to host cell and virus titer. The results showed that the recombinant plasmid pEGFP-MAVS could be expressed in PK-15 cells. And the upregulation MAVS gene can significant inhibition the host cell early replication,and there was a little decrease in virus titer. The study showed that MAVS has some antiviral function,which provided basis for further research of FMDV escape mechanisms to host innate immune.
出处
《中国兽医学报》
CAS
CSCD
北大核心
2017年第3期393-397,共5页
Chinese Journal of Veterinary Science
基金
国家自然科学基金资助项目(31201943)
国家国际科技合作专项资助项目(2011DFA31830)
国家"863"计划资助项目(2011AA10A211-3)