禽呼肠孤病毒感染后SPF鸡关节中Toll样受体的mRNA转录水平变化

Activated expression of toll-like receptors in joints of SPF chickens infected with avian reovirus

禽呼肠孤病毒(avian reovirus,ARV)感染主要引起鸡的病毒性关节炎和免疫抑制等,造成养禽业巨大经济损失。近年来Toll样受体(Toll-like receptors,TLRs)在病原体感染中的作用受到广泛关注。在本试验中,将ARV S1133毒株经足垫注射,接种7日龄SPF鸡,感染后1、3、5、7、14、21、28和35d采集鸡关节样品,利用荧光定量PCR方法检测TLR3、TLR5、TLR7、TLR15和TLR21在关节组织中的mRNA表达水平变化,探讨ARV感染对鸡关节中上述TLRs mRNA表达量的影响。结果表明,ARV可引起,尤其感染早期鸡只出现足垫肿胀的症状,并且关节中这些TLRs在感染后3d显著上调并达到峰值(P<0.05或P<0.01),随后表达量开始下调,在感染后14d表达量显著下调到最低值(P<0.05或P<0.01),然后直至试验结束,恢复至与对照组基本持平。以上结果提示TLR3、TLR5、TLR7、TLR15和TLR21可能参与了ARV感染对鸡关节的炎症过程,本试验结果为进一步阐明禽呼肠孤病毒的致病机理提供了依据。

Avian reovirus （ARV） mainly causes viral arthritis, immune suppression in poultry, re- sulting in huge economic losses in the global poultry industry. Recently much attention has been focused in the function of toll-like receptors （TLRs） in pathogen infection. In this study,ARV in- fected 7-day-old SPF chickens by footpad injection. The mRNA expression levels of TLR3, TLR5, TLRT,TLR15 and TLR21 genes in joints of SPF chickens were detected by the quantitative real- time PCR method. The results showed that ARV infection induced mainly footpad swelling in ARV-infected chicks,and significantly increased the mRNA expression levels of these TLRs, es- pecially at the early infection stage. These TLRs expression reached a maximum value at 3 day post infection （P〈0.05 or P〈0.01）,and subsequently decreased to a minimum value at 14 clay post infection （P〈0.05 or P〈0.01） ,and then returned to the baseline at the late infection stage. These results indicate that TLR3,TLR5 ,TLR7,TLR15 and TLR21 would likely participate in theARV-induced damaging processes in the joints, which would be helpful to further understand ARV pathogenesis.